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| Status |
Public on Nov 09, 2015 |
| Title |
RNA-seq analysis reveals profound changes in transcript profiles between siCon- and siH19-transfected myotubes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We used high throughput sequencing to analyze the transcriptional profiling of myotubes. By comparing the transcriptional profiling of myotubes with or without H19 knockdown,1131 genes or transcripts were found at least 1.5-fold up-regulated in siH19 relative to siCon cells, meanwhile 508 genes or transcripts were found at least 1.5-fold down-regulated in siH19 over siCon cells.
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| |
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| Overall design |
40h following initiation of differentiation, myotubes were transfected with either control siRNA or siH19. 48h later after transfection, total RNA was extracted for library preparation and RNA-seq analysis to compare trancript profiles between siCon and siH19 cells.
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| |
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| Contributor(s) |
Zhang N, Carmichael GG, Huang Y |
| Citation(s) |
26687445 |
| Submission date |
Sep 11, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Na Zhang |
| Organization name |
UConn Health
|
| Department |
Genetics and Genome Sciences
|
| Lab |
Gordon G. Carmichael
|
| Street address |
400 Farmington Ave
|
| City |
Farmington |
| State/province |
CT |
| ZIP/Postal code |
06030 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE73014 |
Genome-wide DNA-methylation and transcriptome change due to lncRNA H19 knockdown in myotubes |
|
| Relations |
| BioProject |
PRJNA295374 |
| SRA |
SRP063608 |
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