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| Status |
Public on Jan 01, 2016 |
| Title |
RNA-seq from animals treated with RNAi against prp-8, prp-6, and prp-31 |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Transcriptome analysis of a population of control animals and RNAi-treated to partially inactivate genes that are homologs to human genes causing Retinitis Pigmentosa. RNA-seq analyses were performed at L3 larval stage in wild type, and smg-1(r861) mutants that have a defective Non-mediated decay pathway. RNA-seq experiments were also performed in adults glp-4(bn2) mutants that lack of germline.
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| Overall design |
synchronized N2 and smg-1(r861) L1 larvae fed for 24 hours at 20 °C with the RNAi clones of prp-6, prp-8, prp-31 and gfp; and 5-day adult glp-4(bn2) worms grown at 25 °C and fed first, for 72 hours with OP50 and next, for 48 more hours with the RNAi clones of prp-8, prp-31 and gfp
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| |
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| Contributor(s) |
Madrigal JC, Aristizábal-Corrales D |
| Citation(s) |
26490224 |
| Submission date |
Sep 11, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Julián Cerón |
| E-mail(s) |
jceron@idibell.cat
|
| Organization name |
IDIBELL
|
| Department |
Cancer and Human Molecular Genetics
|
| Lab |
Modelling human diseases in C. elegans
|
| Street address |
Gran via 199, Hospital Duran i Reynals, planta 3
|
| City |
Hospitalet de Llobregat |
| State/province |
Barcelona |
| ZIP/Postal code |
08908 |
| Country |
Spain |
| |
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| Platforms (1) |
| GPL13657 |
Illumina HiSeq 2000 (Caenorhabditis elegans) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA295401 |
| SRA |
SRP063616 |
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