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| Status |
Public on Sep 12, 2007 |
| Title |
Chromosomal rearrangement interferes with meiotic X-chromosome inactivation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Expression profiling of isolated populations of prepachytene spermatocytes, pachytene spermatocytes and spermatids of males carrying a translocation T(16;17)43H was performed to study the influence of this translocation on gene expression from individual chromosomes.
To evaluate the transcriptional behavior of individual chromosomes in the sterile B10-T43/+ males and their fertile congenic B10-T43/T43 and B10 counterparts, we compared their transcriptomes in sorted populations of pre-pachytene primary spermatocytes, pachytene primary spermatocytes and spermatids.
Keywords: individual genetic characteristics design
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| Overall design |
Populations of pre-pachytene spermatocytes, pachytene spermatocytes and spermatids were isolated from three lines of mice: B10, B10-T43H/T43H and B10-T43H/+. All populations were isolated in duplicates from two animals. Hence, RNA was prepared from 18 samples and was then subject to labeling and hybridization on microarray chips.
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| Contributor(s) |
Ivanek R, Homolka D |
| Citation(s) |
17717048 |
| Submission date |
Mar 19, 2007 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Robert Ivanek |
| Phone |
+41 61 207 35 41
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| Organization name |
University of Basel
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| Department |
Department of Biomedicine
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| Lab |
Bioinformatics
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| Street address |
Hebelstrasse 20
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| City |
Basel |
| ZIP/Postal code |
4053 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL5008 |
Affymetrix GeneChip Mouse Genome 430 2.0 Array [CDF: Mm_ENTREZG_8] |
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| Samples (18)
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| GSM176187 |
C57BL/10J, pre-pachytene spermatocytes, biological rep1 |
| GSM176188 |
C57BL/10J, pre-pachytene spermatocytes, biological rep2 |
| GSM176189 |
C57BL/10J, pachytene spermatocytes, biological rep1 |
| GSM176190 |
C57BL/10J, pachytene spermatocytes, biological rep2 |
| GSM176191 |
C57BL/10J, spermatids, biological rep1 |
| GSM176192 |
C57BL/10J, spermatids, biological rep2 |
| GSM176193 |
C57BL/10J-T43H/T43H, pre-pachytene spermatocytes, biological rep1 |
| GSM176194 |
C57BL/10J-T43H/T43H, pre-pachytene spermatocytes, biological rep2 |
| GSM176195 |
C57BL/10J-T43H/T43H, pachytene spermatocytes, biological rep1 |
| GSM176196 |
C57BL/10J-T43H/T43H, pachytene spermatocytes, biological rep2 |
| GSM176197 |
C57BL/10J-T43H/T43H, spermatids, biological rep1 |
| GSM176198 |
C57BL/10J-T43H/T43H, spermatids, biological rep2 |
| GSM176199 |
C57BL/10J-T43H/+, pre-pachytene spermatocytes, biological rep1 |
| GSM176200 |
C57BL/10J-T43H/+, pre-pachytene spermatocytes, biological rep2 |
| GSM176201 |
C57BL/10J-T43H/+, pachytene spermatocytes, biological rep1 |
| GSM176202 |
C57BL/10J-T43H/+, pachytene spermatocytes, biological rep2 |
| GSM176203 |
C57BL/10J-T43H/+, spermatids, biological rep1 |
| GSM176204 |
C57BL/10J-T43H/+, spermatids, biological rep2 |
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| Relations |
| BioProject |
PRJNA98079 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE7306_RAW.tar |
77.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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