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| Status |
Public on Jan 01, 2008 |
| Title |
Comparison analysis of 2 week old C57BL6J lung exposed to 2 weeks of cigarette smoke compared to age-matched controls |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We hypothesize that gene expression in the cigarette smoke (CS) exposed neonatal lung and age-matched controls will be divergent. CS exposed lung will have divergence of immune response genes and structural genes. The lungs of (6) 2 week old neonatal mice exposed to 2 weeks of CS were compared to the lung of (4) 2 week old age-matched control mice. We utilized microarray analysis to examine transcriptional differences between smoke exposed neonatal lung and age-matched controls.
Keywords: comparative expression profiling
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| Overall design |
This study utilizes microarray analysis to test these hypotheses. Six sets of lungs were harvested from CS exposed mice and four sets of lungs were harvested from age-matched control mice. RNA was isolated and used for global gene expression profiling (Affymetrix Mouse 430 2.0 array). Statistically significant gene expression was determined as a minimum 6 counts of 9 pairwise comparisons, minimum 1.5-fold change, and p < 0.05. Further, Absolute | FC - FC SEM | >= 1.5.
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| Contributor(s) |
McGrath-Morrow S, Biswal S, Rangasamy T, Cho C |
| Citation(s) |
17975176 |
| Submission date |
Mar 19, 2007 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Sharon McGrath-Morrow |
| E-mail(s) |
smorrow@jhmi.edu
|
| Phone |
410-614-5637
|
| Fax |
410-955-1030
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| Organization name |
Johns Hopkins
|
| Department |
Pediatrics
|
| Street address |
200 N.Wolfe St.
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21287 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA97967 |
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