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Series GSE7324 Query DataSets for GSE7324
Status Public on Mar 21, 2007
Title Structural basis for recognition of SMRT/N-CoR by the MYND domain and its contribution to AML1/ETO's activity
Organism Mus musculus
Experiment type Expression profiling by array
Summary To examine the effects of disrupting the AML1/ETO MYND-SMRT interaction with the W692A substitution on AML1/ETO function, the global gene expression profile of mouse bone marrow LSK cells transduced with GFP was compared to that of cells transduced with either wild-type AML1/ETO or AML1/ETO harboring the W692A substitution in the MYND domain. Three independent biological replicates were assessed for both the control (GFP/MigR1) and AML1/ETO (intact MYND-SMRT interaction) conditions, whereas four independent biological replicates were assessed for the W692A (disrupted MYND-SMRT interaction) condition. The three GFP replicates were used to establish a baseline signal for comparison to both the AML1/ETO and W692A samples.
Keywords: genetic modification
 
Overall design Global gene expression profiles of FACS-sorted Lin-Sca1+cKit+ mouse bone marrow cells transduced with empty vector (GFP-MigR1), AML1/ETO, or AML1/ETO with the W692A substitution (W692A).
 
Contributor(s) Speck NA, J Gaudet J, Liu Y, Chen W, Cheney MD, Roudaia L, Cierpicki T, Klet RC, Hartman K, Laue TM, Bushweller JH
Citation(s) 17560331
Submission date Mar 20, 2007
Last update date Aug 29, 2019
Contact name Nancy Asmussen Speck
E-mail(s) Nancy.Speck@dartmouth.edu
Phone (603) 650-1235
Fax (603) 650-1129
Organization name Dartmouth Medical School
Department Biochemistry
Lab Speck Lab
Street address 311 Vail Bldg
City Hanover
State/province NH
ZIP/Postal code 03755
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (10)
GSM176671 GFP-transduced_rep1
GSM176672 GFP-transduced_rep2
GSM176673 GFP-transduced_rep3
Relations
BioProject PRJNA98039

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Supplementary file Size Download File type/resource
GSE7324_RAW.tar 28.1 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data provided as supplementary file

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