|
| Status |
Public on Jan 02, 2016 |
| Title |
Emodin inhibits macrophage alernative activation [Control-IL4-Emodin] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
In order to fully characterize emodin's effects on macrophage alternative activation, peritoneal macrophages were stimulated with IL4 with or without emodin and gene expression was analyzed using a whole genome microarray. Emodin significantly attenuated the IL4 induced changes in a large percentage of genes (60%) through inhibiting multiple signaling pathways. RT-qPCR was used to confirm the results in several genes associated with M2 macrophage activation including: Arg1, Chi3l3, and CD206.
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| |
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| Overall design |
Three-condition, one-color experiment: Vehicle control, IL4 or IL4-Emodin treated periferal WBC PMN samples: 4 biological replicates each.
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| |
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| Contributor(s) |
Altomare D, Iwanowycz S, Fan D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 22, 2015 |
| Last update date |
Feb 02, 2018 |
| Contact name |
Diego Altomare |
| Organization name |
University of South Carolina
|
| Department |
Department of Drug Discovery and Biomedical Sciences
|
| Lab |
Functional Genomics Core
|
| Street address |
715 Sumter Street, Room 617
|
| City |
Columbia |
| State/province |
SC |
| ZIP/Postal code |
29208 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE73311 |
Emodin inhibits macrophage alernative activation |
|
| Relations |
| BioProject |
PRJNA296575 |
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