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| Status |
Public on May 17, 2007 |
| Title |
Comparison of human embryonic stem cell (hESC) derived mesenchymal precursor cell populations |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We have developed efficient protocols for the derivation of mesenchymal precursors from hESCs. While previous protocols were based on mesodermal induction via co-culture of hESCs on OP9 mouse stroma (Barberi et al., PLoS Biology, 2005), our recent work shows the derivation of hESC derived mesenchymal precurors under feeder-free conditions. The data presented here show a large and highly signficant overlap in global gene expression profiles between hESC derived mesenchymal precursors derived under feeder-free conditions with those derived via OP9 co-culure and mesenchymal precurosrs isolated directly from the adult bone marrow.
Keywords: cell type comparison
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| Overall design |
These data compare the gene expression profiles of hESC derived mesenchymal precursors derived via the OP9 protocol, with those obtained under feeder-free conditions and adult bone marrow derived mesenchymal precursors. Three samples of undifferentiated hESCs are used for subtracting genes to define mesenchymal precurosr cell specific signatures (see Barberi et al. Nature Medicine 2007 for details).
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| |
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| Contributor(s) |
Barberi T, Studer L |
| Citation(s) |
17417652 |
| Submission date |
Mar 21, 2007 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Agnes Viale |
| E-mail(s) |
vialea@mskcc.org
|
| Phone |
212 639 3031
|
| Fax |
212 717 3053
|
| Organization name |
Memorial Sloan Kettering Cancer Center
|
| Department |
Molecular Biology
|
| Lab |
Genomics Core Laboratory
|
| Street address |
1275 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
|
| Samples (6)
|
| GSM176732 |
Bone marrow derived mesenchymal precursors (BM_MPC) |
| GSM176734 |
OP9 feeder induced hESC derivec mesenchymal precursors (OP9_hESC_MPC) |
| GSM176738 |
Stroma free hESC derived mesenchymal precursors (SF_hESC_MPC) |
| GSM176743 |
Undifferentiated hESC (hESC_UNDIFF1) |
| GSM176747 |
Undifferentiated human embryonic stem cells (hESC_UNDIFF2) |
| GSM176752 |
Undifferentiated human embryonic stem cells (hESC_UNDIFF3) |
|
| Relations |
| BioProject |
PRJNA98055 |
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