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Series GSE7333 Query DataSets for GSE7333
Status Public on Mar 29, 2007
Title miR-1-2 knockout versus wild-type hearts
Organism Mus musculus
Experiment type Expression profiling by array
Summary microarray was done on Heart tissue from ko and wt
MicroRNAs (miRNAs) are genomically encoded small RNAs used by organisms to regulate the dosage of proteins generated from messenger RNA transcripts. The in vivo requirement of specific miRNAs in mammals is unknown, and reliable prediction of mRNA targets remains problematic. Here, we show that miRNA biogenesis in the mouse heart is essential for cardiogenesis. Furthermore, targeted deletion of the muscle-specific miRNA, miR-1-2, revealed numerous functions in the heart, including regulation of cardiac morphogenesis, electrical conduction, and cell cycle control. Analyses of miR-1 complementary sequences in mRNAs upregulated upon miR-1-2 deletion revealed an enrichment of miR-1 "seed matches" and a strong tendency for potential miR-1 binding sites to be located in physically accessible regions. These findings indicate that subtle alteration of miRNA dosage can have profound consequences in mammals and demonstrate the utility of mammalian loss-of-function models in revealing physiologic miRNA targets.
Keywords: miRNA
Overall design Heart tissues from 3 wild type and 3 miR-1-2 knockout mice at postnatal days 10 were used and total RNA was extracted by Trizol. Expression level was compared between wild type and miR-1-2 knockout mice. The affy package from R/Bioconductor was used to generate RMA values.
Web link
Contributor(s) Zhao Y, Srivastava D
Citation(s) 17397913
Submission date Mar 21, 2007
Last update date Feb 11, 2019
Contact name Yong Zhao
Phone 415-734-2862
Fax 415-355-0960
Organization name The J. David Gladstone Institutes
Department Gladstone Institute of Cardiovascular Disease
Lab Deepak Srivastava
Street address 1650 owens street
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM176756 Wild-type postnatal day 10 rep 1
GSM176757 Knock-out postnatal day 10 rep 1
GSM176758 Wild-type postnatal day 10 rep 2
BioProject PRJNA98057

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7333_RAW.tar 22.0 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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