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| Status |
Public on Sep 24, 2015 |
| Title |
Discovery of progenitor signatures by time series synexpression analysis during Drosophila cell immortalization [RNA-Seq] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To characterize the sequence of events associated with RasV12 immortalization of Drosophila embryonic cells, we generated transcriptional time series during cell line establishment, from primary cultures until passage (P) 19.
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| Overall design |
We generated three transcriptional time series from three cell lines (R1, R4 and R5) by sampling the cultures at successive stages, early (P2-4), intermediate (P4-11), and late (P16-19), characterized by different passage times. Time points for the R1 time-series were: P2, P3, P4, P5, P7, P8, P10, P11, P16, P17 and P19; for the R4 time-series: P2, P3, P4, P5, P6, P7, P9, P11, P12, P16, P17 and P19; and for the R5 time-series: P2, P3, P4, P6, P7, P8, P16, P17 and P19
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| Contributor(s) |
Dequéant M, Fagegaltier D, Hu Y, Spirohn K, Simcox A, Hannon GJ, Perrimon N |
| Citation(s) |
26438832 |
| Submission date |
Sep 23, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Mary-Lee Dequeant |
| E-mail(s) |
mdequeant@genetics.med.harvard.edu, fagegalt@cshl.edu, Greg.Hannon@cruk.cam.ac.uk, perrimon@receptor.med.harvard.edu
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| Phone |
617-432-6855
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| Organization name |
Harvard Medical School
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| Department |
Genetics
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| Lab |
Norbert Perrimon
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| Street address |
77 Avenue Louis Pasteur
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (32)
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| This SubSeries is part of SuperSeries: |
| GSE73354 |
Discovery of progenitor signatures by time series synexpression analysis during Drosophila cell immortalization |
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| Relations |
| BioProject |
PRJNA296785 |
| SRA |
SRP064105 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE73353_RAW.tar |
3.7 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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