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| Status |
Public on Aug 31, 2016 |
| Title |
Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a [gene expression] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Phf5a regulates transcription elongation in mouse embryonic stem cells (ESCs), through regulation of the Paf1 complex.
We used microarrays to detail the global program of gene expression after shControl, shPhf5a, or shPaf1 knockdown in mouse ESCs following 72h knockdown and identified distinct down-regulated and up-regulated genes during this process.
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| Overall design |
Mouse ESCs were transduced with pLKO.1-based backdone lentiviruses expressing shControl, shPhf5a or shPaf1 hairpins, respectively. Cells were selected with puromycin (2μg/mL) for 72h. RNA was extracted using RNeasy kit (Qiagen) and hybridized on Affymetrix microarrays.
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| Contributor(s) |
Strikoudis A |
| Citation(s) |
27749823 |
| Submission date |
Sep 25, 2015 |
| Last update date |
Feb 21, 2018 |
| Contact name |
Alexandros Strikoudis |
| E-mail(s) |
alexandros.strikoudis@med.nyu.edu
|
| Phone |
3475566344
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| Organization name |
NYU Medical Center
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| Department |
Pathology
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| Lab |
Aifantis
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| Street address |
550 First Avenue, Smilow 1303
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE63974 |
Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a |
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| Relations |
| BioProject |
PRJNA297032 |
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