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Series GSE7348 Query DataSets for GSE7348
Status Public on Apr 22, 2007
Title Gene Expression in Naive and Tolerant Macrophages stimulated with LPS
Organism Mus musculus
Experiment type Expression profiling by array
Summary The inflammatory response initiated by microbial products signaling through Toll-like receptors (TLRs) of the innate immune system is essential for host defense against infection. Because inflammation can be harmful to host tissues, the innate response is highly regulated. Negative regulation of TLR4, the receptor for bacterial lipopolysaccharide (LPS), results in LPS tolerance, defined as hyporesponsiveness to repeated stimulation with LPS. LPS tolerance is thought to protect the host from excessive inflammation by turning off TLR4 signal, which then shuts down TLR-induced genes. However, TLR signaling induces hundreds of genes with very different functions. We reasoned that genes with different functions should have different requirements for regulation. Specifically, genes encoding proinflammatory mediators should be transiently inactivated to limit tissue damage, while genes encoding antimicrobial effectors, which directly target pathogens, should remain inducible in tolerant cells to protect the host from infection. Using an in vitro system of LPS tolerance in macrophages, here we show that TLR-induced genes may indeed be divided into two distinct categories based on their functions and regulatory requirements. Further, we show these distinct groups are regulated by gene-specific, and not signal-specific mechanisms.
Keywords: Treatment Comparison
 
Overall design We examined gene expression using affymetrix genechips in 3 groups of murine bone-marrow derived macrophages: Naive (untreated), Naive stimulated with LPS, and Tolerant stimulated with LPS. Two biological replicates were performed for each group.
 
Contributor(s) Foster SL, Hargreaves DC, Medzhitov R
Citation(s) 17538624
Submission date Mar 22, 2007
Last update date Aug 02, 2019
Contact name Simmie L Foster
E-mail(s) simmie.foster@yale.edu
Phone (203)785-6090
Fax (203)785-4461
Organization name Yale University School of Medicine
Department Immunobiology
Lab Medzhitov
Street address 300 Cedar St
City New Haven
State/province CT
ZIP/Postal code 06510
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM177078 Naive-rep2
GSM177079 N+L, rep2
GSM177080 T+L, rep2
Relations
BioProject PRJNA100445

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7348_RAW.tar 23.6 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data provided as supplementary file

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