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Status |
Public on Mar 24, 2007 |
Title |
Early GA response genes in Arabidopsis thaliana |
Organism |
Arabidopsis thaliana |
Experiment type |
Expression profiling by array
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Summary |
The phytohormone GA controls multiple important developmental processes in plants such as germination, elongation growth and flowering time. In this experiment, we look for early GA response genes in 7 day-old light-grown Arabidopsis seedlings. To this end we compare four data sets: (1) a GA biosynthesis mutant ga-1 (SALK_109115) mock treated for 1 hr; (2) a GA biosynthesis mutant ga-1 (SALK_109115) treated for 1 hr with 100 µM GA3; (3) a gid1a-1 gid1b-1 gid1c-2 GA receptor triple mutant mock treated for 1 hr; (4) a gid1a-1 gid1b-1 gid1c-2 GA receptor triple mutant treated for 1 hr with 100 µM GA3. In a comparison of the two ga-1 samples, GA regulated genes can be identified, and the assumption is that bona fide GA regulated genes are not responding in the gid1a-1 gid1b-1 gid1c-2 GA receptor mutant.
Keywords: phytohormone response
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Overall design |
We compare four samples of 7 day-old light-grown growth-medium grown seedlings (3 independent biological replicates each): (1) a GA biosynthesis mutant ga-1 (SALK_109115) mock treated for 1 hr; (2) a GA biosynthesis mutant ga-1 (SALK_109115) treated for 1 hr with 100 µM GA3; (3) a gid1a-1 gid1b-1 gid1c-2 GA receptor triple mutant mock treated for 1 hr; (4) a gid1a-1 gid1b-1 gid1c-2 GA receptor triple mutant treated for 1 hr with 100 µM GA3. In a comparison of the two ga-1 samples, GA regulated genes can be identified, and the assumption is that bona fide GA regulated genes are not responding in the gid1a-1 gid1b-1 gid1c-2 GA receptor mutant.
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Contributor(s) |
Dohmann EM, Nill C, Willige BC, Ghosh S, Zourelidou M, Maier A, Schwechheimer C |
Citation(s) |
17416730 |
Submission date |
Mar 23, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Claus Schwechheimer |
E-mail(s) |
claus.schwechheimer@wzw.tum.de
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Organization name |
TUM
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Department |
Plant Systems Biology
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Street address |
Emil-Ramann-Str. 4
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City |
Freising |
ZIP/Postal code |
85354 |
Country |
Germany |
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Platforms (1) |
GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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Samples (12)
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GSM177119 |
ga-1 (SALK_109115) mock treated for 1 hr, replicate 1 |
GSM177120 |
ga-1 (SALK_109115) mock treated for 1 hr, replicate 2 |
GSM177121 |
ga-1 (SALK_109115) mock treated for 1 hr, replicate 3 |
GSM177122 |
ga-1 (SALK_109115) treated for 1 hr with 100 uM GA3, replicate 1 |
GSM177123 |
ga-1 (SALK_109115) treated for 1 hr with 100 uM GA3, replicate 2 |
GSM177124 |
ga-1 (SALK_109115) treated for 1 hr with 100 uM GA3, replicate 3 |
GSM177125 |
gid1a-1 gid1b-1 gid1c-2 mock treated for 1 hr, replicate 1 |
GSM177126 |
gid1a-1 gid1b-1 gid1c-2 mock treated for 1 hr, replicate 2 |
GSM177127 |
gid1a-1 gid1b-1 gid1c-2 mock treated for 1 hr, replicate 3 |
GSM177128 |
gid1a-1 gid1b-1 gid1c-2 treated for 1 hr with 100 uM GA3, replicate 1 |
GSM177129 |
gid1a-1 gid1b-1 gid1c-2 treated for 1 hr with 100 uM GA3, replicate 2 |
GSM177130 |
gid1a-1 gid1b-1 gid1c-2 treated for 1 hr with 100 uM GA3, replicate 3 |
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Relations |
Affiliated with |
GSE69995 |
BioProject |
PRJNA100421 |
Supplementary file |
Size |
Download |
File type/resource |
GSE7353_RAW.tar |
22.9 Mb |
(http)(custom) |
TAR (of CEL) |
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