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Series GSE7353 Query DataSets for GSE7353
Status Public on Mar 24, 2007
Title Early GA response genes in Arabidopsis thaliana
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary The phytohormone GA controls multiple important developmental processes in plants such as germination, elongation growth and flowering time. In this experiment, we look for early GA response genes in 7 day-old light-grown Arabidopsis seedlings. To this end we compare four data sets: (1) a GA biosynthesis mutant ga-1 (SALK_109115) mock treated for 1 hr; (2) a GA biosynthesis mutant ga-1 (SALK_109115) treated for 1 hr with 100 µM GA3; (3) a gid1a-1 gid1b-1 gid1c-2 GA receptor triple mutant mock treated for 1 hr; (4) a gid1a-1 gid1b-1 gid1c-2 GA receptor triple mutant treated for 1 hr with 100 µM GA3. In a comparison of the two ga-1 samples, GA regulated genes can be identified, and the assumption is that bona fide GA regulated genes are not responding in the gid1a-1 gid1b-1 gid1c-2 GA receptor mutant.

Keywords: phytohormone response
 
Overall design We compare four samples of 7 day-old light-grown growth-medium grown seedlings (3 independent biological replicates each): (1) a GA biosynthesis mutant ga-1 (SALK_109115) mock treated for 1 hr; (2) a GA biosynthesis mutant ga-1 (SALK_109115) treated for 1 hr with 100 µM GA3; (3) a gid1a-1 gid1b-1 gid1c-2 GA receptor triple mutant mock treated for 1 hr; (4) a gid1a-1 gid1b-1 gid1c-2 GA receptor triple mutant treated for 1 hr with 100 µM GA3. In a comparison of the two ga-1 samples, GA regulated genes can be identified, and the assumption is that bona fide GA regulated genes are not responding in the gid1a-1 gid1b-1 gid1c-2 GA receptor mutant.
 
Contributor(s) Dohmann EM, Nill C, Willige BC, Ghosh S, Zourelidou M, Maier A, Schwechheimer C
Citation(s) 17416730
Submission date Mar 23, 2007
Last update date Aug 28, 2018
Contact name Claus Schwechheimer
E-mail(s) claus.schwechheimer@wzw.tum.de
Organization name TUM
Department Plant Systems Biology
Street address Emil-Ramann-Str. 4
City Freising
ZIP/Postal code 85354
Country Germany
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (12)
GSM177119 ga-1 (SALK_109115) mock treated for 1 hr, replicate 1
GSM177120 ga-1 (SALK_109115) mock treated for 1 hr, replicate 2
GSM177121 ga-1 (SALK_109115) mock treated for 1 hr, replicate 3
Relations
Affiliated with GSE69995
BioProject PRJNA100421

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Supplementary file Size Download File type/resource
GSE7353_RAW.tar 22.9 Mb (http)(custom) TAR (of CEL)

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