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| Status |
Public on Apr 05, 2016 |
| Title |
Expression data of endothelial cells from mouse hindbrain and genetic Wnt-medulloblastoma and Shh-medulloblastoma mouse models |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The childhood brain tumour medulloblastoma includes four subtypes with very different prognoses. Here, we show that paracrine signals driven by mutant Beta-Catenin in WNT-medulloblastoma – an essentially curable form of the disease – induce an aberrant fenestrated vasculature that permits the accumulation of high levels of intra-tumoural chemotherapy and a robust therapeutic response. In contrast, SHH-medulloblastoma – a less curable disease subtype – contains an intact blood brain barrier, rendering this tumour impermeable and resistant to chemotherapy. Remarkably, the medulloblastoma-endothelial cell paracrine axis can be manipulated in vivo, altering chemotherapy permeability and clinical response. Thus, medulloblastoma genotype dictates tumour vessel phenotype, explaining in part the disparate prognoses among medulloblastoma subtypes and suggesting an approach to enhance the chemoresponsiveness of other brain tumours.
We used microarrays to detail the global program of gene expression within endothelial cells from normal mouse hindbrain and genetic mouse models of different medulloblastoma subtypes to identify and verify up-regulated and down-regulated genes
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| Overall design |
Endothelial cells were isolated from adult mouse hindbrain and genetic mouse models of Wnt and Shh-medulloblastoma using Cd-144 and Cd-105 antibodies based magnetic sorting. RNA was extracted and used for hybridization on Affymetrix microarrays. We sought to identify changes in endothelial gene expression patterns based on the surrounding microenvironment, so we purified endothelial cells from normal mouse brain or tumors from genetic mouse models. These include the Shh-medulloblastoma model (Ptch+/-; Ink4c -/-) and Wnt-medulloblastoma model (Blbp-Cre; mutant Ctnnb1+/-; p53-/-; mutant Pik3ca +/-)
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| Contributor(s) |
Phoenix T, Gilbertson R |
| Citation(s) |
27050100 |
| Submission date |
Oct 05, 2015 |
| Last update date |
Feb 21, 2018 |
| Contact name |
David Finkelstein |
| E-mail(s) |
david.finkelstein@stjude.org
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| Phone |
9014953931
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| Organization name |
St Jude Children's Research Hospital
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| Department |
Computational Biology
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| Street address |
332 N. Lauderdale St.
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| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (24)
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| GSM1902106 |
adult hindbrain endothelial cells, biological rep1 |
| GSM1902107 |
adult hindbrain endothelial cells, biological rep2 |
| GSM1902108 |
adult hindbrain endothelial cells, biological rep3 |
| GSM1902109 |
adult hindbrain endothelial cells, biological rep4 |
| GSM1902110 |
adult hindbrain endothelial cells, biological rep5 |
| GSM1902111 |
adult hindbrain endothelial cells, biological rep6 |
| GSM1902112 |
adult hindbrain endothelial cells, biological rep7 |
| GSM1902113 |
adult hindbrain endothelial cells, biological rep8 |
| GSM1902114 |
Shh-medulloblastoma endothelial cells, biological rep1 |
| GSM1902115 |
Shh-medulloblastoma endothelial cells, biological rep2 |
| GSM1902116 |
Shh-medulloblastoma endothelial cells, biological rep3 |
| GSM1902117 |
Shh-medulloblastoma endothelial cells, biological rep4 |
| GSM1902118 |
Shh-medulloblastoma endothelial cells, biological rep5 |
| GSM1902119 |
Shh-medulloblastoma endothelial cells, biological rep6 |
| GSM1902120 |
Shh-medulloblastoma endothelial cells, biological rep7 |
| GSM1902121 |
Shh-medulloblastoma endothelial cells, biological rep8 |
| GSM1902122 |
Wnt-medulloblastoma endothelial cells biological rep1 |
| GSM1902123 |
Wnt-medulloblastoma endothelial cells biological rep2 |
| GSM1902124 |
Wnt-medulloblastoma endothelial cells biological rep3 |
| GSM1902125 |
Wnt-medulloblastoma endothelial cells biological rep4 |
| GSM1902126 |
Wnt-medulloblastoma endothelial cells biological rep5 |
| GSM1902127 |
Wnt-medulloblastoma endothelial cells biological rep6 |
| GSM1902128 |
Wnt-medulloblastoma endothelial cells biological rep7 |
| GSM1902129 |
Wnt-medulloblastoma endothelial cells biological rep8 |
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| Relations |
| BioProject |
PRJNA297815 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE73753_RAW.tar |
226.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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