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| Status |
Public on Oct 10, 2015 |
| Title |
Effect of mutant p53 R282W on miRNA expression |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
The H1299 cells stably transfected with mutp53 R282W or the control vector were respectively analyzed by Affymetrix GeneChip miRNA 4.0 microarray with three biological replications.
The labeled RNA was consequently hybridized for sixteen hours to an Affymetrix GeneChip miRNA array according to the product manual. Microarrays were washed and stained in the Affymetrix Fluidics Station 450, and scanned on the Affymetrix G3000 GeneArray Scanner.
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| |
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| Overall design |
Controlled experiments with 3 repeats for each condition.
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| |
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| Contributor(s) |
Xu J |
| Citation(s) |
26840456 |
| Submission date |
Oct 09, 2015 |
| Last update date |
May 02, 2017 |
| Contact name |
Jie Xu |
| E-mail(s) |
xujieletter@gmail.com
|
| Organization name |
Shanghai Institute for Digestive Diseases
|
| Department |
Shanghai Renji Hospital
|
| Lab |
State Key Laboratory for Oncogenes and Related Genes
|
| Street address |
Shandong Rd 145
|
| City |
Shanghai |
| ZIP/Postal code |
200001 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL19117 |
[miRNA-4] Affymetrix Multispecies miRNA-4 Array |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA298290 |
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