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| Status |
Public on May 04, 2016 |
| Title |
Gene Regulation by microRNA-16 forced expression in ErbB-2 positive murine breast cancer cells. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
miR-16 is a potent tumor suppressor in ErbB-2 positive breast cancer. The primary objetive of this study was to identify previously uncharacterized putative mRNA targets of miR-16 in ErbB-2 postitive breast cancer cells. To achieve our objective we studied the effects of exogenously expressed miR-16 on the gene expression profile of primary cultures of ErbB-2 positive murine breast cancer tumors. The C4HD tumor line displays high levels of estrogen receptor and progesterone receptor, overexpresses ErbB-2 and ErbB-3, exhibits low ErbB-4 levels, and lacks EGF-R expression.
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| Overall design |
Primary cultures of the C4HD murine breast tumor were transfected with a miR-16 precursor (pre-miR-16) or a scrambled sequence (pre-miR-Control) that does not form any known mammalian miRNA. Three biological replicates were obtained for each experimental condition.
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| Contributor(s) |
Elizalde PV, Cidlowski JA, Venturutti L, Rivas MA |
| Citation(s) |
27157613 |
| Submission date |
Oct 09, 2015 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Patricia Virginia Elizalde |
| E-mail(s) |
patriciaelizalde@ibyme.conicet.gov.ar
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| Phone |
+54 11 4783-2869
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| Organization name |
Instituto de Biología y Medicina Experimental
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| Lab |
Molecular Mechanisms of Carcinogenesis
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| Street address |
Vuelta de Obligado 2490
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| City |
Ciudad Autónoma de Buenos Aires |
| State/province |
Ciudad Autónoma de Buenos Aires |
| ZIP/Postal code |
C1428ADN |
| Country |
Argentina |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA298360 |
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