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Series GSE7396 Query DataSets for GSE7396
Status Public on Aug 01, 2008
Title TfRc Dependent Gene Expression in Embryonic Renal Cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary We investigated the ability of transferrin receptor1 (TfRc) knockout cells to populate different domains of the developing kidney by using a chimeric approach. The TfRc cells developed into all segments of the developing nephron, but there was a relative exclusion from the ureteric bud and a positive bias towards the stromal compartment. Here we conducted a microarray analysis of differential gene expression between TfRc deficient and wild type (wt) cells in chimeric embryonic kidneys derived from embryos created by blastocyst injection of wt blastocysts with TfRc-/- green fluorescent protein-expressing (GFP+) embryonic stem cells.
Keywords: cell type comparison, genetic modification, iron signaling
 
Overall design Following blastocyst injection of 3-5 TfRc-/- GFP+ embryonic stem cells in wt blstocysts, we harvested the chimeric kidney at day E15.5 and separated the TfRc-/- GFP+ cell population from wt GFP- cell population by using fluorescence activated cell sorting. Biotinlyated cRNA was prepared from the isolated cells and hybridized to Mouse Genome 430 2.0 Arrays (Affymetrix) according to standard protocols.
 
Contributor(s) Li J, Paragas N, Schmidt-Ott KM, Chen X, Barasch J
Citation(s) 19154717
Submission date Mar 29, 2007
Last update date Feb 11, 2019
Contact name Jonathan Barasch
E-mail(s) jmb4@columbia.edu
Organization name Columbia University
Street address 630 West 168th St
City New York
State/province NY
ZIP/Postal code 10032
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (4)
GSM177573 wildtype (GFP-negative) kidney cells, biological rep1
GSM177574 wildtype (GFP-negative) kidney cells, biological rep2
GSM177575 Tfrc-/- (GFP-positive) kidney cells, biological rep1
Relations
BioProject PRJNA97885

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Supplementary file Size Download File type/resource
GSE7396_RAW.tar 13.5 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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