NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE74276 Query DataSets for GSE74276
Status Public on Feb 14, 2017
Title Gene expression profiling of plasmacytoid dendritic cells from cutaneous lymph nodes
Organism Mus musculus
Experiment type Expression profiling by array
Summary Plasmacytoid dendritic cells wre isolated from cutaneous lymph nodes of control C57BL/6 mice and used for microarray analysis.
 
Overall design Plasmacytoid dendritic cells were isolated from cutaneous lymph nodes (CLNs) as previously described (Henri et al. 2010). Briefly, CLNs were cut into small pieces and digested for 20 min at room temperature with a mixture of type II collagenase (Worthington Biochemical) and DNase I (Sigma-Aldrich). The resulting cell suspension was treated with 5 mM EDTA to disrupt DC-T cell conjugates. After eliminating undigested material, light-density cells were enriched by centrifugation on an Optiprep solution (d = 1.32 g/mL, Abcys).
 
Contributor(s) Henri S, Chelbi R, Dalod M, Malissen B
Citation(s) 26966045
Submission date Oct 22, 2015
Last update date Mar 04, 2019
Contact name Marc DALOD
E-mail(s) dalod@ciml.univ-mrs.fr
Organization name CIML
Street address Parc Scientifique et Technologique de Luminy, 163 avenue de Luminy, Case 906, 13288 Marseille cedex 9
City MARSEILLE
ZIP/Postal code 13009
Country France
 
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (3)
GSM1916134 pDC.CLN_1
GSM1916135 pDC.CLN_2
GSM1916136 pDC.CLN_3
Relations
BioProject PRJNA299628

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE74276_RAW.tar 11.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap