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Series GSE7430 Query DataSets for GSE7430
Status Public on Apr 04, 2007
Title Investigate the role of Wnt/beta-catenin signaling in development of the exocrine pancreas
Organism Mus musculus
Experiment type Expression profiling by array
Summary beta-catenin is an essential mediator of canonical Wnt signaling and a central component of the cadherin-catenin epithelial adhesion complex. Dysregulation of beta-catenin expression has been described in pancreatic neoplasia. Newly published studies have suggested that beta-catenin is critical for normal pancreatic development although these reports reached somewhat different conclusions. In addition, the molecular mechanisms by which loss of beta-catenin affects pancreas development are not well understood. The goals of this study then were; 1] to further investigate the role of beta-catenin in pancreatic development using a conditional knockout approach and 2] to identify possible mechanisms by which loss of beta-catenin disrupts pancreatic development. A Pdx1-cre mouse line was used to delete a floxed beta-catenin allele specifically in the developing pancreas, and embryonic pancreata were studied by immunohistochemistry and microarray analysis.

Keywords: Time course
 
Overall design Parameter: embryonic pancreas from wild type and Pdx1Cre/+; beta-cateninflox/flox
Sample type: RNA
Source name: Embryonic day 14.6 and 16.5 pancreas
Organism: Mus Musculus
Strain: Derived from an intercross of Pdx1Cre/+ ; beta-cateninflox/+ mice.
Extracted molecule: Total RNA
In the study, we hybridized RNA from Embryonic day 14.6 and 16.5 pancreas of wild type (WT) control and Pdx1Cre/+; beta-cateninflox/flox beta-catenin null in the pancreas) embryonic pancreas to Affymetrix MOE430 2.0 GeneChip® arrays containing 45,101 well characterized mouse genes/ESTs.
 
Contributor(s) Wells JM, Aronow BJ
Citation(s) 17222338
Submission date Apr 02, 2007
Last update date Feb 11, 2019
Contact name Bruce J Aronow
E-mail(s) bruce.aronow@chmcc.org
Phone 513-636-4865
Organization name Cincinnati Children's Hospital Medical Center
Street address
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (12)
GSM177393 14.5_KO_540C
GSM177394 16.5_KO_591D
GSM177395 16.5_WT_628G
Relations
BioProject PRJNA97951

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7430_RAW.tar 45.2 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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