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Status |
Public on Dec 22, 2018 |
Title |
Amyloid-β42-induced neurodegeneration enhances stem cell plasticity and neurogenesis in adult zebrafish brain |
Organism |
Danio rerio |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
To analyze the effects of Ab42-mediated neurodegeneration in adult zebrafish brain, we generated an Ab42 deposition model and analyzed the transcriptome of stem cells and parenchymal cells.
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Overall design |
6 months old Tg(her4.1:EGFP) adult fish brains were injected with CPP or AB42 using CVMI method (Kizil etal, 2013). 48 hpi brains were dissected, dissocited and non-GFP (GFP-N) and GFP positive (GFP-P) cells were sorted with FACS. Following total RNA isolation, samples were sequenced with triple replicates on Illumina HiSeq 2000 platform (75 bp end-read).
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Contributor(s) |
Bhattarai P, Cosacak MI, Dahl A, Kizil C |
Citation(s) |
27760324, 34685728 |
Submission date |
Oct 23, 2015 |
Last update date |
Oct 27, 2021 |
Contact name |
Caghan Kizil |
E-mail(s) |
ck2893@cumc.columbia.edu
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Phone |
4752570197
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Organization name |
Columbia University Irving Medical Center
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Street address |
650 W 168th St.
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City |
New York |
State/province |
NY |
ZIP/Postal code |
10032 |
Country |
USA |
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Platforms (1) |
GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
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Samples (12)
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Relations |
BioProject |
PRJNA299681 |
SRA |
SRP065201 |