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Series GSE74469 Query DataSets for GSE74469
Status Public on May 04, 2016
Title Effects of bedrest and hypoxia on human skeletal muscle miRNA expression
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary The present study concerns the effects of 21 days of sustained recumbence (bedrest) and hypoxia, alone and in combination, on skeletal muscle microRNA expression.
14 male subjects participated in 3 experimental campaigns in a counterbalanced fashion: normoxic bedrest (NBR), hypoxic bedrest (HBR) and hypoxic ambulatory confinement (HAMB), both hypoxic conditions with FO2 = 0.141 and PIO2 = 90 ± 0.4 mmHg, equivalent to an altitude of ≈ 4000 m). Each intervention (bedrest or ambulatory confinement) lasted 21 days, and the interventions were separated by a 4-month wash-out/recovery period. The order in which each subject undertook the interventions is denoted 'Intervention_order' Throughout both bedrest interventions, each subject remained in a horizontal position at all times. He was allowed one pillow for the head and to occasionally lean on an elbow while eating or being transferred to a gurney. Muscular exercise was prohibited. During the HAMB confinement, each subject was allowed to move freely within the hypoxic area. To mimic a normal level of physical activity subjects performed 30 minutes of low-to-moderate-intensity exercise twice daily. Muscle samples from Vastus lateralis.
 
Overall design Biospy material sufficient for anlaysis was avialable for 72 observations, another 4 were excluded due to technical problems with the array hybridisation, leaving in total 68 observations.
 
Contributor(s) Rullman E
Citation(s) 27117806
Submission date Oct 29, 2015
Last update date May 02, 2017
Contact name Eric Rullman
E-mail(s) eric.rullman@gmail.com
Phone +46858586777
Organization name Karolinska Institutet
Department Laboratory Medicine
Lab Clinical Physiology
Street address C1:82 Karolinska University Hospital, Huddinge
City Stockholm
ZIP/Postal code 14186
Country Sweden
 
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (68)
GSM1921195 S1_Pre_hypoxic ambulatory confinement (HAMB)
GSM1921196 S1_Post_hypoxic ambulatory confinement (HAMB)
GSM1921197 S1_Pre_hypoxic bedrest (HBR)
Relations
BioProject PRJNA300517

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE74469_RAW.tar 59.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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