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| Status |
Public on Apr 27, 2017 |
| Title |
The role of Hoxa9 and Meis1 in development of acute myeloid leukemia |
| Platform organism |
synthetic construct |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
OBJECTIVE: MEIS1, a HOX cofactor, collaborates with multiple HOX proteins, such as HOXA9, to accelerate the onset of acute myeloid leukemia (AML) through largely unknown molecular mechanisms. To further resolve these mechanisms, we conducted a structure-function analysis of Meis1 and miRNA expression profiling, in the context of Hoxa9 leukemogenesis. RESULTS: We show, in a murine bone marrow transplantation model, that the homeodomain of Meis1 is required for leukemogenic collaboration with Hoxa9. miRNA expression profiling of a Hoxa9 preleukemic cell line transduced with wild-type or Meis1 homeodomain mutant reveal deregulation of multiple miRNA including a set not previously implicated as Meis1 targets.
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| Overall design |
Murine bone marrow cells transduced with Hoxa9-GFP + empty MIY vector were compared to Hoxa9+Meis1 cells or Hoxa9+Meis1 with deleted homeodomain (DHD) cells and cultured for three or four weeks before harvest for miRNA expression array. Four independent experiments were performed for each of the three different conditions included in the study. Cells from all samples were also transplanted into lethally irradiated mice to test for their transforming and leukemic potential.
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| Contributor(s) |
Palmqvist L |
| Citation(s) |
28399410 |
| Submission date |
Nov 02, 2015 |
| Last update date |
Jul 27, 2018 |
| Contact name |
Lars Palmqvist |
| E-mail(s) |
lars.palmqvist@clinchem.gu.se
|
| Organization name |
University of Gothenburg
|
| Department |
Institute of Biomedicine
|
| Lab |
Department of Laboratory Medicine
|
| Street address |
Sahlgrenska University Hospital
|
| City |
Gothenburg |
| ZIP/Postal code |
41345 |
| Country |
Sweden |
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| Platforms (1) |
| GPL16384 |
[miRNA-3] Affymetrix Multispecies miRNA-3 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA301939 |
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