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| Status |
Public on Nov 05, 2015 |
| Title |
hESC MIXL1+ MIXL1- microarray |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Microarray analysis of isolated hES cells from day 3 of cardiac differentiation was used to identify differences between MIXL1eGFP+ and MIXL1eGFP- transcriptomes. We identified 6,757 differentially regulated genes, of which 2,520 were upregulated ≥2-fold in the eGFP+ (MIXL1+) mesoderm population
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| Overall design |
A stencil differentiation protocol was used to isolate mesodermal cells based on GFP expression from the MIXL1 locus. Microarray analysis of isolated cells from day 3 of differentiation was used to identify differences between MIXL1eGFP+ and MIXL1eGFP- transcriptomes.
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| Contributor(s) |
Skelton RJ, Brady B, Sahoo D, Abilez OJ |
| Citation(s) |
26771355 |
| Submission date |
Nov 04, 2015 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Rhys John Powling Skelton |
| E-mail(s) |
rskelton@student.unimelb.edu.au
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| Organization name |
UCLA
|
| Department |
Eli and Edythe Broad Stem Cell Research Center
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| Lab |
Ardehali Lab
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| Street address |
675 Charles E Young Dr S, Room 3645
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| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE74713 |
Isolation of highly enriched cardiac mesoderm from differentiating human embryonic stem cells |
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| Relations |
| BioProject |
PRJNA301126 |
