|
| Status |
Public on Aug 01, 2016 |
| Title |
Comparation the mRNA Expression Profile of the MV4-11 Sorafenib Resistant Cells and the Parental Cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Our gene set analysis of MV4-11-R versus MV4-11 indicated decreased depolarization of mitochondria and mitochondrial membrane, mitochondrial dysfunction and anti-apoptosis as other top ranked molecular or cellular functions of differential gene sets. expression of most genes encoding glycolytic enzymes was up-regulated in MV4-11-R cells we revealed a metabolic alteration in sorafenib-resistant cell lines with mitochondrial respiration deficiency, leading to substantial decrease of mitochondria-derived ATP generation and a significant increase in glycolytic activity to maintain sufficient ATP production. Our study revealed a metabolic signature of sorafenib resistance and indicated that increase of glycolytic activity including upregulation of major glycolytic enzymes may be viewed as a marker for early detection of sorafenib resistance in AML patients with FLT3/ITD mutation and glycolytic inhibitors warrant further investigation as alternative therapeutic agents for sorafenib-resistant cells
|
| |
|
| Overall design |
Sorafenib resistant cells MV411-R VS. parental MV4-11 cells. Biological replicates: 3 control replicates, 3 treated replicates.
|
| |
|
| Contributor(s) |
Huang A, Hu Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 04, 2015 |
| Last update date |
Jan 09, 2018 |
| Contact name |
Amin Huang |
| Organization name |
Sun Yat-sen University Cancer Center
|
| Street address |
651 Dongfengdong East Rd
|
| City |
Guangzhou |
| ZIP/Postal code |
510060 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA301124 |
Less...
More...