NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE7468 Query DataSets for GSE7468
Status Public on Nov 04, 2007
Title Gene Regulation by HDAC7 in Thymic Selection
Organism Mus musculus
Experiment type Expression profiling by array
Summary Histone deacetylase 7 (HDAC7) is highly expressed in CD4+/CD8+ thymocytes and functions as a signal-dependent repressor of gene transcription during T cell development. In this study, we express HDAC7 mutant proteins in a T cell line and use DNA microarrays to identify transcriptional targets of HDAC7 in T cells. Gene expression changes are compared to differential gene expression profiles associated with positive and negative thymic selection. This analysis reveals that HDAC7 regulates an extensive set of genes that are differentially expressed during both positive and negative thymic selection. Many of these genes play important functional roles in positive and negative selection, primarily via coupling between antigen receptor and downstream signaling events.
Keywords: microarray gene expression profiling, comparison of perturbation of HDAC7 gene function with differential expression during thymic selection
 
Overall design DO11.10 cells were transduced with different viruses encoding mutants of HDAC7, MEF2D, or Nur77. All microarrays were hybridized with untreated DO11.10 cells in one channel and transdced/treated cells in the other. Hybridizations are done with 4-12 replicates. DO11.10 TCR-transgenic thymocytes were also compared to MHC-deficient thymocytes and DO11.10 TCR-transgenic thymocytes from animals that had been injected with antigenic peptide at different timepoints prior to harvest of thymocytes.
 
Contributor(s) Kasler HG, Verdin EM
Citation(s) 17470548
Submission date Apr 05, 2007
Last update date Jan 17, 2013
Contact name Eric Verdin
E-mail(s) everdin@buckinstitute.org
Phone (415) 209-2250
Organization name Buck Institute for Research on Aging
Department NA
Lab Verdin
Street address 8001 Redwood Blvd
City Novato
State/province CA
ZIP/Postal code 94945
Country USA
 
Platforms (1)
GPL5064 Gladstone Core Operon Mouse V 2.0 spotted array
Samples (74)
GSM180706 slide 14-1 cy3 untreated cy5 MSCV
GSM180707 Slide 15-1 cy 3 untreated cy 5 MSCV
GSM180708 Slide 14-2 cy 3 untreated cy 5 MSCV
Relations
BioProject PRJNA100267

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7468_RAW.tar 153.1 Mb (http)(custom) TAR (of GPR)

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap