NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE74824 Query DataSets for GSE74824
Status Public on Mar 17, 2016
Title Expression data of Cohesin-deficient, human cord blood-derived, hematopoietic CD34 cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Decreased levels of Cohesin proteins increases the amount of primitive hematopoietic stem and progenitor cells in an in vitro setting.
We used microarrays to detail the changes in global transcription programme to understand the underlying mechanisms behind this phenotype. Of note, we discover that hematopoietic stem cell-specific genes are upregulated in cells with decreased Cohesin levels.
 
Overall design CD34 positive cells were isolated from human cord blood (not older than 24h), lentivirally transduced with shRNA targeting different members of the Cohesin complex. In order to focus on immediate changes, transduced cells were sorted 36 hours post transduction and analyzed.
 
Contributor(s) Galeev R, Larsson J
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Nov 09, 2015
Last update date Apr 20, 2018
Contact name Roman Galeev
E-mail(s) roman.galeev@med.lu.se
Phone +46736240268
Organization name Lund University
Street address Sölvegatan 17
City Lund
ZIP/Postal code SE-22184
Country Sweden
 
Platforms (1)
GPL13158 [HT_HG-U133_Plus_PM] Affymetrix HT HG-U133+ PM Array Plate
Samples (33)
GSM1936026 Non-transduced cells, biological rep1
GSM1936027 Non-transduced cells, biological rep2
GSM1936028 Non-transduced cells, biological rep3
Relations
BioProject PRJNA301614

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE74824_RAW.tar 71.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap