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Series GSE7503 Query DataSets for GSE7503
Status Public on Aug 01, 2007
Title Effects of JNK on TGF-beta responses of mouse B lymphoma derived A20 cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary TGF-beta plays multiple functions in a board range of cellular responses such as proliferation, differentiation, motility and survival by activating several cellular signaling pathways, including Smads and MAP kinases (Erk, JNK and p38). In particular, TGF-beta can activate pro- or anti-apoptotic signals depending on the target cells. We found that blockage of JNK activation sensitized mouse B lymphoma derived A20 cells to TGF-beta-induced apoptosis. These results suggest that TGF-beta activate JNK to inhibit the activation of death signal that is simultaneously activated by TGF-beta.
We used microarrays to gain insight into the effects of JNK inhibition on gene expression in TGF-b-stimulated A20 cells and identified JNK-dependent TGF-beta inducible genes.
Keywords: time course
Overall design The following six samples were prepared: untreated A20 cells (non-stimulated, DMSO): A20 cells cultured with SP600125 for 24 h (non-stimulated, SP600125): A20 cells stimulated with TGF-beta for 12 h (TGF-beta 12 h, DMSO) and 24 h (TGF-beta 24 h, DMSO): and A20 cells stimulated with TGF-beta in the presence of SP600125 for 12 h (TGF-beta 12 h, SP600125) and 24 h (TGF-beta 24 h, SP600125), respectively. Total RNA was prepared and hybridized to the Affymetrix Mouse Genome 430 2.0 array. Genes whose expression was increased by more than 2-fold at either 12 or 24 h after TGF-beta stimulation were identified as TGF-beta inducible genes. Amongst them, we identified genes whose induction levels were reduced by more than 75% by co-treatment with the JNK inhibitor SP600125.
Contributor(s) Ohgushi M, Yamamoto T, Nishida E, Yonehara S
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Submission date Apr 12, 2007
Last update date Feb 11, 2019
Contact name shin yonehara
Organization name Graduate School of Biostudies, Kyoto University
Street address Yoshida Konoe-cho, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8501
Country Japan
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM181858 non-stimulated_DMSO
GSM181859 non-stimulated_SP600125
GSM181860 TGF-beta12h_DMSO
BioProject PRJNA100489

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Supplementary file Size Download File type/resource
GSE7503_RAW.tar 20.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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