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Status |
Public on Jul 31, 2017 |
Title |
The long noncoding RNA expression profiles of paroxysmal atrial fibrillation identified by microarray analysis |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array Non-coding RNA profiling by array
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Summary |
The purpose of the present study was to identify aberrantly expressed lncRNAs in pAF and predict their potential functions. We recruited three pAF patients undergoing radiofrequency catheter ablation into our study. Peripheral blood leukocytes (PBL) and coronary sinus blood leukocytes (CSBL) were harvested from pAF patients at the same time during the operation. LncRNAs and mRNAs profiles in leukocytes were detected by microarray between pAF patients and controls. We identified 2095 lncRNAs and 1584 mRNAs differentially expressed between pAF patients and controls. Furthermore, expressions of lncRNAs and mRNAs in leukocytes were also measured between peripheral blood (PB) and coronary sinus blood (CSB). The 866 lncRNAs and 528 mRNAs were shown to be aberrantly expressed between CSBL and PBL in all three pAF patients. Bioinformatic analyses (lncRNA classification and subgroup, gene ontology analysis, pathway analysis and gene co-expression network construction) were performed for differentially expressed lncRNAs and mRNAs. Lastly, we confirmed seven aberrant lncRNAs and five dysregulated mRNAs by qRT-PCR. Our results demonstrated the aberrantly expressed lncRNAs and mRNAs in pAF and these lncRNAs/mRNAs may exert a vital role in pAF biology.
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Overall design |
To explore the potential involvement of lncRNAs in paroxysmal atrial fibrillation(pAF), we firstly conducted lncRNAs and mRNAs profiling in leukocytes by microarray between pAF patients and controls. Then, expressions of lncRNAs/mRNAs in leukocytes were detected between peripheral blood (PB) and coronary sinus blood (CSB) in all three pAF patients.
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Contributor(s) |
Su Y, Yue Y, Yang S |
Citation missing |
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Submission date |
Nov 17, 2015 |
Last update date |
Mar 12, 2018 |
Contact name |
Ying Su |
Organization name |
Capital Medical University affiliated Beijing Shijitan Hospital
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Street address |
Tieyi Rd. No.10, Yangfangdian, Haidian district
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City |
Beijing |
ZIP/Postal code |
100038 |
Country |
China |
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Platforms (1) |
GPL16956 |
Agilent-045997 Arraystar human lncRNA microarray V3 (Probe Name Version) |
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Samples (9)
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GSM1942514 |
ExVivoBlood_paroxysmal atrial fibrillation_coronary sinus blood_rep1 |
GSM1942515 |
ExVivoBlood_paroxysmal atrial fibrillation_coronary sinus blood_rep2 |
GSM1942516 |
ExVivoBlood_paroxysmal atrial fibrillation_coronary sinus blood_rep3 |
GSM1942517 |
ExVivoBlood_paroxysmal atrial fibrillation_peripheral blood_rep1 |
GSM1942518 |
ExVivoBlood_paroxysmal atrial fibrillation_peripheral blood_rep2 |
GSM1942519 |
ExVivoBlood_paroxysmal atrial fibrillation_peripheral blood_rep3 |
GSM1942520 |
ExVivoBlood_control_peripheral blood_rep1 |
GSM1942521 |
ExVivoBlood_control_peripheral blood_rep2 |
GSM1942522 |
ExVivoBlood_control_peripheral blood_rep3 |
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Relations |
BioProject |
PRJNA302440 |