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| Status |
Public on Apr 18, 2007 |
| Title |
Allergen-stimulated CD4+ T cells from hen's egg allergic patients |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Allergen-stimulated T cells from hen’s egg-allergic children were analyzed to identify genes that are specifically up-regulated in these cells.
Keywords: disease state analysis
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| Overall design |
PBMCs from three hen’s egg allergic children and two non-allergic children were cultured in RPMI 1640 supplemented with 10% autologous plasma for 16 hours with or without hen’s egg white allergen (allergen concentration10 mg/ml). To focus on specific T cells reacting with HEW, cells bearing CD4 antigen were isolated from cultured PBMCs using a Magnetic cell sorter（Miltenyi Biotec, Bergisch Gladbach, Germany）after CD14 positive cell depletion. Total RNA was extracted from these CD4 positive cells with an RNeasy mini-kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instruction.
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| Contributor(s) |
Nakajima Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 13, 2007 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Nakajima Yoichi |
| E-mail(s) |
ynakaji@fujita-hu.ac.jp
|
| Phone |
+81 562 93 9251
|
| Fax |
+81 562 95 2216
|
| Organization name |
Fujita Health University
|
| Department |
Pediatrics
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| Lab |
allergy
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| Street address |
1-98 Dengakugakubo Kutsukake
|
| City |
Toyoake |
| State/province |
Aichi |
| ZIP/Postal code |
470-1192 |
| Country |
Japan |
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| Platforms (1) |
| GPL1708 |
Agilent-012391 Whole Human Genome Oligo Microarray G4112A (Feature Number version) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA100461 |
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