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Series GSE7528 Query DataSets for GSE7528
Status Public on Jan 02, 2008
Title Gene expression of Wt vs CYP26A1-/- murine ES cells treated with control or 100 nM RA for 8 or 72 hr.
Organism Mus musculus
Experiment type Expression profiling by array
Summary The goal of this study was to identify genes that are differentially expressed after genetic deletion of both alleles of the Cyp26a1 gene in murine embryonic stem cells. Cyp26a1 codes for the CYP26A1 enzyme which metabolizes RA to polar RA metabolites, such as 4-oxo-RA and 4-OH-RA. CYP26A1-/- ES cells do not metabolize RA within 48 hours of RA treatment while in Wt ES cells, polar RA metabolites are already detectable by 8 hr. In addition, the absence of CYP26A1 enzyme increases intracellular RA levels. By gene microarray analysis, we wanted to identify genes that would be affected by the lack of the Cyp26a1 gene.
Keywords: cell type comparison, time course
 
Overall design Wt and CYP26A1-/- ES cells were treated with control+LIF for 8 hours, and this was repeated 3 times. Wt and CYP26A1-/- ES cells were treated with 100 nM RA +LIF for 8 hours, and this was repeated 3 times. Wt and CYP26A1-/- ES cells were treated with 100 nM RA +LIF for 72 hours, and this was repeated 2 times.
 
Contributor(s) Langton S, Gudas LJ
Citation(s) 18241852
Submission date Apr 16, 2007
Last update date Feb 11, 2019
Contact name Simne Langton
Phone 212-746-6262
Organization name Weill Cornell Medical College
Department Pharmacology
Lab Lorraine J. Gudas
Street address 1300 York Ave, E405
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (18)
GSM182238 WtES_Control+LIF_8hr
GSM182240 WtES_Control_minusLIF_8hr
GSM182243 WtES_RA+LIF_8hr
Relations
BioProject PRJNA99397

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7528_RAW.tar 307.8 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data provided as supplementary file

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