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| Status |
Public on Dec 02, 2015 |
| Title |
GFRα1+, NGN3+ and KIT+ spermatogonia of adult mouse testes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
In adult mouse testis, undifferentiated spermatogonia include GFRα1+ and NGN3+ populations. The former mainly act as self-renewing stem cells that support steady-state spermatogenesis. Whilst, the latter are primed for differentiation, but retain the potential of self-renewal and contribute to regeneration and post-transplantation colony formation (Nakagawa et al. science .2010; Hara et al. cell stem cell. 2014). And, NGN3+ cells can differentiate to KIT+ cells, which are also mentioned differentiated spermatogonia, by retinoic acid signal. The gene expression profiles of GFRα1+, NGN3+ and KIT+ spermatogonia, as well as the whole testis samples, were investigated here by microarray (Ikami et al. development. 2015).
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| Overall design |
GFRα1+ spermatogonia were isolated by FACS from cell suspensions prepared from the testes of GFRα1-EGFP knock-in (Enomoto et al. development. 2000). NGN3+ and KIT+ spermatogonia were isolated from Ngn3-EGFP transgenic (Yoshida et al. Dev Biol. 2004) mice as GFP+/KIT- and GFP+/KIT+ fractions, respectively. Each GFRα1+ or KIT+ fraction was collected from a single individual, while each NGN3+ fraction was pooled from two mice. In addition, whole testes samples were prepared from WT littermates of GFRα1-EGFP knock-in mouse. All animals were sacrificed at the age of 10-11 weeks old.
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| Citation(s) |
25858458 |
| Submission date |
Dec 01, 2015 |
| Last update date |
Feb 02, 2018 |
| Contact name |
Shosei Yoshida |
| E-mail(s) |
shosei@nibb.ac.jp
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| Organization name |
National Institute for Basic Biology
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| Department |
Division of Germ Cell Biology
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| Street address |
5-1,Higashiyama, Myodaiji-cho
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| City |
Okazaki |
| State/province |
Aichi prefecture |
| ZIP/Postal code |
444-8787 |
| Country |
Japan |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA304491 |
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