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| Status |
Public on Dec 01, 2015 |
| Title |
Analysis of altered miRNA expression profiles in the genetic hypercalciuric stone-forming (GHS) rat compared to normal SD rat |
| Platform organism |
synthetic construct |
| Sample organism |
Rattus norvegicus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Urolithiasis is a common disease to human beings, and idiopathic hypercalciuria (IH) is an important risk factor of calcium urolithiasis, previous studies strongly suggested that the decreased tubular Ca2+ reabsorption played a key role of hypercalciuria. However,the molecular mechanism of IH-urolithiasis formation is still not completely elucidated. GHS rat is regarded as an ideal animal model of calcium urolithiasis, reveals many identical pathophysiologic characteristics with IH patients . We analyzed miRNA expression profiles of the kidney of GHS rat in order to find out the target genes and signaling pathways in the pathogenesis of IH.
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| Overall design |
The colony of GHS rats were created by the selective breeding of male and female SD rats with the highest 24 hour urine excretions (doi: 10.3390/ijms160716313). In the 28 generation, GHS rats have stabilized excreted significant higher level of urine calcium than wild-type normal SD rats, and reveals many identical pathophysiologic characteristics with IH patients, such as normal serum Ca2+, hypercalciuria, elevated intestinal Ca2+ resorption and a tendency to lose calcium from the bone. This present study examined the miRNAs expression of three pairs of GHS rats’ kidneys with normal SD rats by microarrays.
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| Contributor(s) |
Lu Y, Wang S |
| Citation(s) |
27069814 |
| Submission date |
Dec 01, 2015 |
| Last update date |
May 02, 2017 |
| Contact name |
Yuchao LU |
| E-mail(s) |
luyuchao@hust.edu.cn
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| Organization name |
Tongji Hospital of Tongji medical college, Huazhong University of Science &Technology
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| Department |
Urology
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| Street address |
1095# Jiefang Avenue
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| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430030 |
| Country |
China |
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| Platforms (1) |
| GPL19117 |
[miRNA-4] Affymetrix Multispecies miRNA-4 Array |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE75543 |
miRNA and mRNA profiles in the genetic hypercalciuric stone-forming (GHS) rat compared to normal SD rat |
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| Relations |
| BioProject |
PRJNA304499 |
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