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Series GSE75727 Query DataSets for GSE75727
Status Public on Feb 02, 2016
Title The PGC-1α/ERRα Axis Represses One-Carbon Metabolism and Promotes Sensitivity to Anti-Folate Therapy in Breast Cancer [Microarray expression]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Reprogramming of cellular metabolism plays a central role in fuelling malignant transformation, and AMPK as well as the PGC-1α/ERRα axis are key regulators of this process. Intersection of gene expression and binding event datasets in breast cancer cells shows that activation of AMPK significantly increases the expression of PGC-1α/ERRα and promotes the binding of ERRα to its cognate sites. Unexpectedly, the data also reveal that ERRα, in concert with PGC-1α, negatively regulates the expression of several one-carbon metabolism genes resulting in substantial perturbations in purine biosynthesis. This PGC-1α/ERRα-mediated repression of one-carbon metabolism promotes the sensitivity of breast cancer cells and tumors to the anti-folate drug methotrexate. These data implicate the PGC-1α/ERRα axis as a core regulatory node of folate cycle metabolism and further suggest that activators of AMPK could be used to modulate this pathway in cancer.
We used microarrays to detail the global program of gene expression following AMPK activation by AICAR in BT474 breast cancer cells.
 
Overall design BT474 breast cancer cells were treated for 24h with vehicle or the AMPK activator AICAR and RNA was then purified for microarray analysis. Experiments were performed in duplicate for each condition.
 
Contributor(s) Audet-Walsh E, Papadopoli D, St-Pierre J, Giguère V
Citation(s) 26804918
Submission date Dec 05, 2015
Last update date Mar 25, 2019
Contact name Etienne Audet-Walsh
E-mail(s) etienne.audet-walsh@crchudequebec.ulaval.ca
Organization name Université Laval
Department Molecular Medicine
Street address 2705 boulevard Laurier
City Québec City
State/province QC
ZIP/Postal code G1V 4G2
Country Canada
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (4)
GSM1964812 BT474_1day_vehicle_rep1
GSM1964813 BT474_1day_vehicle_rep2
GSM1964814 BT474_1day_AICAR_rep1
This SubSeries is part of SuperSeries:
GSE75877 The PGC-1α/ERRα Axis Represses One-Carbon Metabolism and Promotes Sensitivity to Anti-Folate Therapy in Breast Cancer
Relations
BioProject PRJNA305189

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE75727_RAW.tar 21.2 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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