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Series GSE7596 Query DataSets for GSE7596
Status Public on Feb 15, 2008
Title AKT regulates de novo induction of Foxp3
Organism Mus musculus
Experiment type Expression profiling by array
Summary The CD4+Foxp3+ regulatory T cells play an essential role in maintaining tolerance via their suppressive function on conventional T cells. The intracellular signaling pathways that regulate Foxp3 expression are largely unknown. In this study we describe a novel inhibitory role for AKT in regulating de novo induction of Foxp3 both in vivo and in vitro. A constitutively active allele of AKT significantly diminished TGF-รข induced Foxp3 induction via a rapamycin-sensitive pathway, establishing a role for the AKT-mTOR axis in Treg cells. Moreover, the observed impairment in Foxp3 induction was paralleled by a selective downmodulation of the imparted Treg transcriptional signature highlighting the importance of the balance of intracellular signals in Treg differentiation . Our results provide a basis for further elucidation of molecular mechanisms that regulate Foxp3 induction and identify AKT as an important negative regulator of this process.
Keywords: Cell population comparison
Overall design All gene expression profiles were obtained from highly purified T cell populations sorted by flow cytometry. To reduce variability, cells from multiple mice were pooled for sorting, and three replicates were generated for all groups. RNA from 0.5-2.5 x 105 cells was amplified, labeled, and hybridized to Affymetrix M430v2 microarrays. Raw data were preprocessed with the RMA algorithm in GenePattern, and averaged expression values were used for analysis.
Contributor(s) Haxhinasto S, Mathis D, Benoist C
Citation(s) 18283119
Submission date Apr 24, 2007
Last update date Feb 11, 2019
Contact name CBDM Lab
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM176890 AktTGF, rep1
GSM176891 GFPTGF, rep1
GSM180510 AktTGF, rep2
BioProject PRJNA100163

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Supplementary file Size Download File type/resource
GSE7596_RAW.tar 24.5 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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