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| Status |
Public on Dec 19, 2015 |
| Title |
Genome-wide mapping of retinoblastoma (Rb) binding sites in prostate cancer cell lines VCaP and LNCaP |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Although well characterized as a transcriptional activator, androgen receptor (AR) can also function as a direct transcriptional repressor in prostate cancer cells. The major targets of the AR repressive function are genes mediating DNA synthesis. In this study, we found that AR was recruited to the majority of these DNA synthesis genes and rapidly repressed their transcription. This direct AR mediated repression was enhanced in prostate cancer cells expressing higher levels of AR, and was mediated by recruitment of hypophosphorylated retinoblastoma protein (Rb).
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| Overall design |
Examination of Rb binding in 4 hours DHT treated prostate cancer cell lines VCaP and LNCaP
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| Contributor(s) |
Han D, Gao S, Cai C |
| Citation(s) |
27760327 |
| Submission date |
Dec 18, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Dong Han |
| E-mail(s) |
dong.han@umb.edu
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| Phone |
6172873447
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| Organization name |
Umass Boston
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| Department |
Biology-CPCT
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| Lab |
Changmeng Cai
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| Street address |
100 Morrissey Blvd
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02125 |
| Country |
USA |
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| Platforms (1) |
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| Samples (2) |
| GSM1974981 |
Rb ChIP-Seq in VCaP cells treated with 10nM DHT for 4 hours |
| GSM1974982 |
Rb ChIP-Seq in LNCaP cells treated with 10nM DHT for 4 hours |
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| Relations |
| BioProject |
PRJNA306425 |
| SRA |
SRP067555 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE76141_RAW.tar |
1.3 Mb |
(http)(custom) |
TAR (of BED, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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