|
| Status |
Public on Apr 28, 2016 |
| Title |
Sp7/Osterix is restricted to bone-forming vertebrates where it acts as a Dlx co-factor in osteoblast specification [ChIP-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Sp7/Osterix is a master regulator of osteoblast specification. To identify the Sp7-mediated gene regulatory network in osteoblasts, we performed Sp7 ChIP-seq on primary mouse calvarial osteoblasts comparing the DNA binding profile with the transcriptional profile of Sp7-positive osteoblasts. Analysis of these identified a network of Sp7 regulated osteoblast targets and provides a new insight into the mode of Sp7 action in osteoblast. To further study for the Sp7 mode, we performed ChIP-seq for Sp1, Sp7, Dlx5, as a potential Sp7 partner identified in this study and mutated Sp7 which has mutations in the zinc finger domain, by using in vitro system with a pre-osteoblast mouse cell line, MC3T3E1.
|
| |
|
| Overall design |
ChIP-seq experiments with primary osteoblasts and an osteoblast cell line were performed.
|
| |
|
| Contributor(s) |
Hojo H, Ohba S, He X, Lai LP, McMahon AP |
| Citation(s) |
27134141 |
| Submission date |
Dec 20, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Hironori Hojo |
| E-mail(s) |
hojo@tetrapod.t.u-tokyo.ac.jp
|
| Phone |
+81-3-5841-1427
|
| Organization name |
The University of Tokyo
|
| Department |
Center for Disease Biology and Integrative Medicine
|
| Lab |
Clinical Biotechnology
|
| Street address |
7-3-1 Hongo
|
| City |
Bunkyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
113-8655 |
| Country |
Japan |
| |
|
| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (15)
|
|
| This SubSeries is part of SuperSeries: |
| GSE76187 |
Sp7/Osterix is restricted to bone-forming vertebrates where it acts as a Dlx co-factor in osteoblast specification |
|
| Relations |
| BioProject |
PRJNA306570 |
| SRA |
SRP067608 |
Less...
More...