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| Status |
Public on Nov 27, 2016 |
| Title |
Transcriptome analysis of Drosophila melanogaster third instar larval ring glands points to novel functions and uncovers a cytochrome p450 required for development |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
Third-party reanalysis
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| Summary |
In Drosophila melanogaster larvae the ring gland is a control center that orchestrates major developmental transitions. It is a composite organ, consisting of the prothoracic gland, the corpus allatum and the corpora cardiaca, each of which synthesizes and secretes a different hormone. Until now, the ring gland’s broader developmental roles beyond endocrine secretion have not been explored. RNA sequencing and analysis of a new transcriptome resource from D. melanogaster wandering third instar larval ring glands has provided a fascinating insight into the diversity of developmental signalling in this organ. We have found strong enrichment of expression of two gene pathways not previously associated with the ring gland: immune response and fatty acid metabolism. We have also uncovered strong expression for many uncharacterized genes. Additionally, RNA interference against ring gland-enriched cytochrome p450s Cyp6u1 and Cyp6g2 produced a lethal ecdysone deficiency and a juvenile hormone deficiency respectively, flagging a critical role for these genes in hormone synthesis. This transcriptome provides a valuable new resource for investigation of roles played by the ring gland in governing insect development.
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| Overall design |
Investigation of genome-wide expression in the third instar larval ring gland of two strains: Celera and Armenia-14. Three biological replicates were examined. For differential expression analysis, duplicate RNA-seq reads for the third instar larval central nervous system were obtained from the modMINE database (accession: SRX029398) [i.e., third-party re-analysis].
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| Contributor(s) |
Christesen D, Yang Y, Somers J, Robin C, Sztal T, Batterham P, Perry T |
| Citation(s) |
27974438 |
| Submission date |
Dec 23, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Trent Perry |
| E-mail(s) |
trentp@unimelb.edu.au
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| Organization name |
The University of Melbourne
|
| Department |
Biosciences
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| Lab |
Batterham
|
| Street address |
30 Flemington Road
|
| City |
Parkville |
| State/province |
Victoria |
| ZIP/Postal code |
3010 |
| Country |
Australia |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA306859 |
| SRA |
SRP067745 |
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