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Series GSE76330 Query DataSets for GSE76330
Status Public on Dec 25, 2015
Title Global analysis of genes regulated by canonical Wnt pathway
Platform organism Rattus norvegicus
Sample organism Rattus norvegicus albus
Experiment type Expression profiling by array
Summary Up-regulation of canonical Wnt signaling has been implicated in liver fibrosis and cancer.
To determine canonical Wnt target genes potentially implicated in these diseases, we performed microarray analysis of culture-activated rat hepatic stellate cells (HSCs) treated with canonical Wnt inhibitors, DKK-1, ICG-001, or FJ9.
Overall design DKK-1, ICG-001, and FJ9 treatment of HSCs: Fully activated primary rat HSCs (cultured for 7 days on plastic dish with 10% FBS containing low glucose DMEM) were treated with: 1) adenovirus expressing DKK-1 (Ad.DKK-1) vs. Ad.GFP (control) at a multiplicity of infection (MOI) of 200 for 24 h in low glucose DMEM with 5% FBS and antibiotics, and then maintained for an additional 24 h in serum-free medium; 2) ICG-001 (10 µM) vs. vehicle for 24 h in serum-free medium; or 3) FJ9 (200 µM) vs. vehicle for 24 h in serum-free medium. No replicates were used. Instead, each of the 6 conditions (3 treatments and 3 respective controls)/samples consisted of a pool of RNA from 3 to 4 rats.
Contributor(s) Lai K, Tsukamoto H
Citation(s) 28143772
Submission date Dec 24, 2015
Last update date Jul 11, 2019
Contact name Hidekazu Tsukamoto
Organization name University of Southern California
Department Pathology
Lab Southern California Research Center for ALPD & Cirrhosis
Street address 1333 San Pablo Street MMR414
City Los Angeles
State/province CA
ZIP/Postal code 90089
Country USA
Platforms (1)
GPL6247 [RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version]
Samples (6)
GSM1981360 HSCs, Dkk1 treatment
GSM1981361 HSCs, Control for Dkk1
GSM1981362 HSCs, ICG-001 treatment
BioProject PRJNA306922

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE76330_RAW.tar 24.4 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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