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Series GSE76508 Query DataSets for GSE76508
Status Public on Jan 21, 2016
Title Comparison of expression data between control, Atg7, Bdh2, Atg7 and Bdh2 dual morphant zebrafish embryos
Organism Danio rerio
Experiment type Expression profiling by array
Summary Bdh2 morphants displayed mitochondrial dysfunction and degradation by autophagy, as well as hypohemoglobinization. Suppression of Atg7 expression in Bdh2 morphants diminished mitochondrial clearance and partly corrected their hemoglobinization defect.
Overall design Total RNAs from control, Atg7, Bdh2, Atg7 and Bdh2 dual morphant zebrafish embryos were isolated at 72 hpf using the RNeasy Mini kit (Qiagen). RNA samples were processed as per manufacturer’s instructions (Affymetrix GeneChip Expression Analysis Technical Manual, Affymetric, Inc., Santa Clara, CA, USA) at Case Comprehensive Cancer Center Microarray Core Facility. Experiments were performed in triplicate for control, Atg7, Atg7 and Bdh2 dual morphant zebrafish embryos, and in singlet for Bdh2 morphant zebrafish embryos.
Contributor(s) Liu Z, Devireddy L, Davuluri G, Sakaguchi T
Citation(s) 26929344
Submission date Jan 04, 2016
Last update date Jan 25, 2018
Contact name Zhuoming Liu
Organization name Case Western Reserve University
Street address 2103 Cornell Road
City Cleveland
State/province OH
ZIP/Postal code 44106
Country USA
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (10)
GSM2027265 control morphant, rep 1
GSM2027266 Atg7 morphant, rep 1
GSM2027267 Atg7 and Bdh2 dual morphant, rep 1
BioProject PRJNA307595

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE76508_RAW.tar 20.6 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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