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| Status |
Public on Jan 21, 2016 |
| Title |
Comparison of expression data between control, Atg7, Bdh2, Atg7 and Bdh2 dual morphant zebrafish embryos |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
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| Summary |
Bdh2 morphants displayed mitochondrial dysfunction and degradation by autophagy, as well as hypohemoglobinization. Suppression of Atg7 expression in Bdh2 morphants diminished mitochondrial clearance and partly corrected their hemoglobinization defect.
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| Overall design |
Total RNAs from control, Atg7, Bdh2, Atg7 and Bdh2 dual morphant zebrafish embryos were isolated at 72 hpf using the RNeasy Mini kit (Qiagen). RNA samples were processed as per manufacturer’s instructions (Affymetrix GeneChip Expression Analysis Technical Manual, Affymetric, Inc., Santa Clara, CA, USA) at Case Comprehensive Cancer Center Microarray Core Facility. Experiments were performed in triplicate for control, Atg7, Atg7 and Bdh2 dual morphant zebrafish embryos, and in singlet for Bdh2 morphant zebrafish embryos.
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| Contributor(s) |
Liu Z, Devireddy L, Davuluri G, Sakaguchi T |
| Citation(s) |
26929344 |
| Submission date |
Jan 04, 2016 |
| Last update date |
Jan 25, 2018 |
| Contact name |
Zhuoming Liu |
| Organization name |
Case Western Reserve University
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| Street address |
2103 Cornell Road
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| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44106 |
| Country |
USA |
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| Platforms (1) |
| GPL1319 |
[Zebrafish] Affymetrix Zebrafish Genome Array |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA307595 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE76508_RAW.tar |
20.6 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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