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Series GSE76509 Query DataSets for GSE76509
Status Public on Jan 21, 2016
Title Comparison of expression data between control and Bdh2 morphant zebrafish embryos, as well as Bdh2 morphant zebrafish embryos expressing ectopic Bdh2
Organism Danio rerio
Experiment type Expression profiling by array
Summary Bdh2 morphants displayed mitochondrial dysfunction and degradation by autophagy, which could be restored by ectopic Bdh2 overexpression.
 
Overall design Total RNAs from control and Bdh2 morphant zebrafish embryos, as well as Bdh2 morphant zebrafish embryos expressing ectopic Bdh2 were isolated at 72 hpf using the RNeasy Mini kit (Qiagen). RNA samples were processed as per manufacturer’s instructions (Affymetrix GeneChip Expression Analysis Technical Manual, Affymetric, Inc., Santa Clara, CA, USA) at Case Comprehensive Cancer Center Microarray Core Facility. Experiments were performed in duplicate for control and Bdh2 morphant zebrafish embryos, and in singlet for Bdh2 morphant zebrafish embryos expressing ectopic Bdh2.
 
Contributor(s) Liu Z, Devireddy L, Davuluri G, Sakaguchi T
Citation(s) 26929344
Submission date Jan 04, 2016
Last update date Jan 25, 2018
Contact name Zhuoming Liu
Organization name Case Western Reserve University
Street address 2103 Cornell Road
City Cleveland
State/province OH
ZIP/Postal code 44106
Country USA
 
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (5)
GSM2027275 control morphant, rep 1
GSM2027276 Bdh2 morphant, rep 1
GSM2027277 Bdh2 morphant, rep 2
Relations
BioProject PRJNA307594

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE76509_RAW.tar 10.7 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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