|
Status |
Public on Jan 12, 2016 |
Title |
Gene expression data from lung of mice infected with H5N1 or H1N1 virus |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
|
Summary |
To find the different host response during H5N1 and H1N1 infection, we have employed whole genome microarray expression profiling as a discovery platform to identify genes differentially expressed in mouse lungs infected by H5N1 and H1N1 virus. BALB/c mice were infected with live H5N1 virus , live H1N1 virus, or inactivated H5N1 virus or allantoic fluid (AF) for 24 h.
|
|
|
Overall design |
Mice were intra-tracheally inoculated with 106 TCID50 of live H5N1 virus or an identical volume of inactivated H5N1 virus , 106 TCID50 of live H1N1 virus, or allantoic fluid (AF). After 24 hours, lungs were taken for RNA extraction and hybridization on Agilent microarrays.H5N1 and H1N1 virus induced gene expression in mouse was measured. This experiment was performed with 3 mice in each group.
|
|
|
Contributor(s) |
Zhang Y, Tang J, Yang N, Sun Y, Jiang C |
Citation(s) |
27354218 |
Submission date |
Jan 11, 2016 |
Last update date |
Jan 12, 2017 |
Contact name |
Yanli Zhang |
Organization name |
Chinese Academy of Medical Sciences, Peking Union Medical College
|
Lab |
State Key Laboratory of Medical Molecular Biology
|
Street address |
No.5 Dongdansantiao
|
City |
Beijing |
State/province |
BEIJING |
ZIP/Postal code |
100005 |
Country |
China |
|
|
Platforms (1) |
GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
|
Samples (5)
|
|
Relations |
BioProject |
PRJNA308453 |