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Status |
Public on Feb 01, 2016 |
Title |
Expression data from polarized adult human macrophages |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Myeloid cells are prominent cellular constituents of the CNS. Under physiologic conditions, these include microglia within the parenchyma and systemic compartment derived macrophages localized to the perivascular spaces. Defining the relative distribution and functions of microglia versus blood-derived macrophages in the CNS parenchyma under pathologic conditions remains a challenge due to limitations in being able to distinguish these cell types. Approaches to distinguishing microglia and macrophages in experimental models include use of chimeric and parabiotic animals and molecular genetic techniques to selectively differentially label or delete a specific cell type. The current report will compare gene expression of human microglia and macrophages under distinct states of activation or “polarization” and relate these to their roles in tissue injury and protection /repair in the central nervous system (CNS). We used Affymetrix Human Gene HT 2.0 microarrays to compare whole transcriptomes of microglia and macrophages under defined polarization conditions.
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Overall design |
Macrophages isolated from adult human peripheral blood of 3 donors were exposed to different polarization conditions: M0 (MSCF), Mtgf (MCSF+TGFβ), M1 (GMCSF+IFNƴ+LPS), M2a (MCSF+IL4+IL13) and M2c (MCSF+IL10+IL4+IL13) over a 7 day period.
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Contributor(s) |
Healy L, Perron G |
Citation(s) |
26962228, 28303091 |
Submission date |
Jan 11, 2016 |
Last update date |
Jul 12, 2019 |
Contact name |
Luke Michael Healy |
E-mail(s) |
healylm@tcd.ie
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Phone |
15148151916
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Organization name |
Montreal Neurological Institute
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Department |
Neurology and Neurosurgery
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Lab |
Dr. Jack Antel
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Street address |
3801 University Street
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City |
Montreal |
State/province |
Quebec |
ZIP/Postal code |
H3A 2B4 |
Country |
Canada |
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Platforms (1) |
GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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Samples (15)
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This SubSeries is part of SuperSeries: |
GSE76737 |
Expression data from polarized adult human microglia and macrophages |
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Relations |
BioProject |
PRJNA308470 |