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Series GSE76736 Query DataSets for GSE76736
Status Public on Feb 01, 2016
Title Expression data from polarized adult human macrophages
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Myeloid cells are prominent cellular constituents of the CNS. Under physiologic conditions, these include microglia within the parenchyma and systemic compartment derived macrophages localized to the perivascular spaces. Defining the relative distribution and functions of microglia versus blood-derived macrophages in the CNS parenchyma under pathologic conditions remains a challenge due to limitations in being able to distinguish these cell types. Approaches to distinguishing microglia and macrophages in experimental models include use of chimeric and parabiotic animals and molecular genetic techniques to selectively differentially label or delete a specific cell type. The current report will compare gene expression of human microglia and macrophages under distinct states of activation or “polarization” and relate these to their roles in tissue injury and protection /repair in the central nervous system (CNS).
We used Affymetrix Human Gene HT 2.0 microarrays to compare whole transcriptomes of microglia and macrophages under defined polarization conditions.
 
Overall design Macrophages isolated from adult human peripheral blood of 3 donors were exposed to different polarization conditions: M0 (MSCF), Mtgf (MCSF+TGFβ), M1 (GMCSF+IFNƴ+LPS), M2a (MCSF+IL4+IL13) and M2c (MCSF+IL10+IL4+IL13) over a 7 day period.
 
Contributor(s) Healy L, Perron G
Citation(s) 26962228, 28303091
Submission date Jan 11, 2016
Last update date Jul 12, 2019
Contact name Luke Michael Healy
E-mail(s) healylm@tcd.ie
Phone 15148151916
Organization name Montreal Neurological Institute
Department Neurology and Neurosurgery
Lab Dr. Jack Antel
Street address 3801 University Street
City Montreal
State/province Quebec
ZIP/Postal code H3A 2B4
Country Canada
 
Platforms (1)
GPL16686 [HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version]
Samples (15)
GSM2036961 macrophages_M0_1
GSM2036962 macrophages_M0_2
GSM2036963 macrophages_M0_3
This SubSeries is part of SuperSeries:
GSE76737 Expression data from polarized adult human microglia and macrophages
Relations
BioProject PRJNA308470

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE76736_RAW.tar 121.4 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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