|
| Status |
Public on Jan 12, 2016 |
| Title |
Global gene expression profiling of Jmjd6- and Jmjd4-depleted mouse NIH3T3 fibroblasts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Emerging evidence suggests Jumonji domain-containing proteins are epigenetic regulators in diverse biological processes including cellular differentiation and proliferation. RNA interference-based analyses combined with gene expression profiling can effectively characterize the cellular functions of these enzymes. We found that the depletion of Jumonji domain-containing protein 6 (Jmjd6) and its paralog protein Jumonji domain-containing protein 4 (Jmjd4) individually by small hairpin RNAs (shRNAs) slowed cell proliferation of mouse NIH3T3 fibroblasts. We subsequently performed gene expression profiling on both Jmjd6- and Jmjd4-depleted mouse NIH3T3 fibroblasts using the Affymetrix GeneChip Mouse Exon 1.0 ST Array.
|
| |
|
| Overall design |
We anaylzed control shRNA-treated cells, Jmjd6- and Jmjd4- depleted cells using Affymetrix Mouse Exon 1.0 ST platform. Two biological replicates were performed (total 6 samples). Array data was processed by Affymetrix Expression Console.
|
| |
|
| Contributor(s) |
Hu Y, Imbalzano AN |
| Citation(s) |
27071056 |
| Submission date |
Jan 11, 2016 |
| Last update date |
May 29, 2020 |
| Contact name |
Yu-Jie Hu |
| E-mail(s) |
Yu-Jie.Hu@umassmed.edu
|
| Organization name |
UMass Medical School
|
| Department |
Cell & Developmental Biology
|
| Street address |
55 Lake Avenue North
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01655 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL6096 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version] |
| GPL6193 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [probe set (exon) version] |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA308504 |
Less...
More...