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Series GSE7676 Query DataSets for GSE7676
Status Public on Jun 30, 2008
Title Changes in gene expression following Protocadherin 12 knockout
Organism Mus musculus
Experiment type Expression profiling by array
Summary Protocadherin 12 (Pcdh12) is a transmembrane adhesive protein with homophilic adhesive properties and expressed in endothelial cells, the glycogen trophoblast cells of the placenta, and the mesangial cells of kidney glomeruli. Pcdh12-deficient mice are alive although they show alterations in placenta development.
To reveal Pcdh12-associated pathways, 45,000 - Affymetrix probe arrays were used on Pcdh12 knockout and control placentas.
Keywords: Comparative gene knockout results
 
Overall design To identify genes whos expression was modified by Pcdh12 deficiency, we examined global gene expression profiles of whole E12.5 knockout placentas (Pcdh12 -l-) and their wild-type (WT – Pcdh12+l+ ) littermates, using the Affymetrix Murine Genome 430 2.0 microarrays. Gestation day 12 was chosen as it is the period at which placentas already formed distinct layers, but organogenesis is still very active. Total RNA was extracted from five Pcdh12 knockout embryos and five normal placentas. RNA were tested for integrity on Agilent 2100 BioAnalyzer, prior to cRNA synthesis and chip hybridization (performed by the Institute of Genetics and Molecular and Cellular Biology, Strasbourg, France).
 
Contributor(s) Rampon C, Bouillot S, Climescu-Haulica A, Prandini M, Cand F, Vandenbrouck Y, Huber P
Citation(s) 18477666
Submission date May 01, 2007
Last update date Feb 11, 2019
Contact name Philippe HUBER
E-mail(s) phuber@cea.fr
Organization name Inserm-CEA
Department iRTSV
Lab Lab U882 – Vascular Physiopathology
Street address 17, Rue des martyrs
City GRENOBLE
ZIP/Postal code 38054
Country France
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (10)
GSM185954 pcdh12-deficient mice rep1
GSM185955 pcdh12-deficient mice rep2
GSM185956 pcdh12-deficient mice rep3
Relations
BioProject PRJNA99717

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7676_RAW.tar 54.7 Mb (http)(custom) TAR (of CEL, EXP)
Raw data provided as supplementary file

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