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Series GSE76795 Query DataSets for GSE76795
Status Public on Feb 28, 2017
Title Defining stem cell dynamics and migration during wound healing in mouse skin epidermis
Organism Mus musculus
Experiment type Expression profiling by array
Summary Wound healing is essential to repair the skin after injury. In the epidermis, distinct stem cells (SCs) populations contribute to wound healing. However, how SCs balance proliferation, differentiation and migration to repair a wound remains poorly understood. Here we show the cellular and molecular mechanisms that regulate wound healing in mouse tail epidermis. Using a combination of proliferation kinetics experiments and molecular profiling, we identify the gene signatures associated with proliferation, differentiation and migration in different regions surrounding the wound. Functional experiments show that SC proliferation, migration and differentiation can be uncoupled during wound healing. Lineage tracing and quantitative clonal analysis reveal that, following wounding, progenitors divide more rapidly, but conserve their homeostatic mode of division, leading to their rapid depletion whereas SCs become active, giving rise to new progenitors that expand and repair the wound. These results have important implications for tissue regeneration, acute and chronic wound disorders.
 
Overall design To micro dissect the different regions surrounding the wound, at D4 and D7 post wound, one punch of 4mm in diameter was done, around the wound 3mm in diameter, to remove the leading edge zone and a punch of 6mm of diameter was used to remove the proliferative hub. Another piece of skin was taken, as control, from a region far from the wounded area. The two replicates samples at each time point were a pull of 5 CD1 mice. For each sample, 300 basal cells were FACS sorted and used for RNA extraction and hybridization on Affymetrix microarrays.
 
Contributor(s) Aragona M, Dekoninck S, Blanpain C
Citation(s) 28248284
Submission date Jan 12, 2016
Last update date Jun 26, 2019
Contact name Cedric Blanpain
Organization name Université Libre De Bruxelles
Street address route de Lennik, 808
City Bruxelles
ZIP/Postal code B-1070
Country Belgium
 
Platforms (1)
GPL11180 [HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate
Samples (12)
GSM2038006 Leading edge basal cells_ D4 post wound_Biological rep1
GSM2038007 Leading edge basal cells_ D4 post wound_Biological rep2
GSM2038008 Proliferative hub basal cells_D4 post wound_Biological rep1
Relations
BioProject PRJNA308599

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE76795_RAW.tar 26.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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