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Series GSE7694 Query DataSets for GSE7694
Status Public on Jun 11, 2007
Title Cannabinoid receptor double knockout mice (Cnr1 -/- /Cnr2 -/-) in CHS model
Organism Mus musculus
Experiment type Expression profiling by array
Summary We evaluated cutaneous contact hypersensitivity (CHS) in Cnr1-/-/Cnr2-/- animals using the obligate contact allergen 2,4-dinitrofluorobenzene (DNFB), which generates a specific cutaneous T-cell mediated allergic response upon repeated allergen contact. Allergic contact dermatitis affects about 5% of men and 11% of women in industrialized countries and is one of the leading causes for occupational diseases. In an animal model for cutaneous contact hypersensitivity we show that mice lacking both known cannabinoid receptors display exacerbated allergic inflammation. In contrast, fatty acid amide hydrolase deficient mice, which have increased levels of the endocannabinoid anandamide, displayed reduced allergic responses in the skin. Cannabinoid receptor antagonists exacerbated whereas receptor agonists attenuated allergic inflammation. These results demonstrate a protective role of the endocannabinoid system in contact allergy in the skin, and suggest a novel target for therapeutic intervention.
Keywords: Strain (Wt versus Ko) and disease state (DNFB treated versus control).
 
Overall design Three wildtype mice (Wt) and three Cnr1-/-/Cnr2-/- (Ko) mice were used. Contact hypersensitivity was determined always at the right ears, which therefore were treated with DNFB (Tr). Left ears of mice were kept untreated and served as control ears (C). A total of 12 hybridizations were performed (2 strains x 2 treatments X 3 biological replicates) in this experiment.
 
Contributor(s) Karsak M, Gaffal E, Wang-Eckhardt L, Tüting T, Zimmer A
Citation(s) 17556587
Submission date May 03, 2007
Last update date Feb 11, 2019
Contact name Meliha Karsak
Organization name University of Bonn
Department Molecular Psychiatry
Street address Sigmund-Freud-Str. 25
City Bonn
ZIP/Postal code 53127
Country Germany
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (12)
GSM186666 C57BL/6J female number 1, untreated control ear (#1_Wt-C-3)
GSM186667 C57BL/6J female number 2, untreated control ear (#2_Wt-C-4)
GSM186668 C57BL/6J female number 3, untreated control ear (#3_Wt-C-7)
Relations
BioProject PRJNA99455

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7694_RAW.tar 193.8 Mb (http)(custom) TAR (of CEL, CHP, EXP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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