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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 01, 2016 |
| Title |
Compare proB cells from WT, Tp53-/-, Lnk-/-, Tp53-/-Lnk-/- mice and Tp53-/-Lnk-/-B-ALL |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The adaptor protein LNK (SH2B3) has emerged as an important protein in regulating B cell development B cell leukemia. Loss-of-function mutations in LNK (SH2B3) are found in Philadelphia chromosome–like acute lymphoblastic leukemia (Ph-like ALL), but how LNK regulates normal B cell development or promotes leukemogenesis remains unclear. We found that combined loss of Lnk and tumor suppressors Tp53 in mice triggers a highly aggressive and transplantable precursor B-ALL. This study aims to investigate the molecular mechanism by which LNK regulates B-ALL development. We performed expression profiling of bone marrow proB progenitors from WT, Tp53-/-, Lnk-/- and preleukemic healthy Tp53/Lnk double knockout (DKO) mice, as well as leukemic bone marrow cells from DKO mice that have developed B-ALL. Results suggest that Tp53-/-Lnk-/- B-ALLs display similar gene expression profiles to human Ph-like B-ALLs, suggesting this model for preclinical and molecular studies.
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| Overall design |
B220+CD19+CD43+AA4.1+IgM-NK1.1-Ly6c- bone marrow proB cells were double sorted from WT, Tp53-/-, Lnk-/- and preleukemic healthy Tp53/Lnk double knockout (DKO) mice, as well as leukemic bone marrow cells from DKO mice that have developed B-ALL. RNA was isolated using miRNeasy kit from QIAGEN and processed using the NuGEN Pico kit. The microarray analysis was performed at the PENN Molecular Profiling/Genomics Facility using GeneChip Mouse Gene 2.0ST array (Affymetrix).
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| Contributor(s) |
Cheng Y, Chikwava K, Wu C, Zhang H, Bhagat A, Glasgow S, Pei D, Choi JK, Tong W |
| Citation(s) |
26974155 |
| Submission date |
Jan 18, 2016 |
| Last update date |
Feb 21, 2018 |
| Contact name |
Wei Tong |
| E-mail(s) |
tongw@email.chop.edu
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| Phone |
267-626-0930
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| Organization name |
Children's Hospital of Philadelphia, and University of Pennsylvania School of Medicine
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| Department |
Pediatrics
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| Lab |
Tong
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| Street address |
316A Abramson Bldg., 3615 Civic Center Blvd.
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19004 |
| Country |
USA |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (15)
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| GSM2041255 |
Tp53-/- proB cells, biological replicate 1 |
| GSM2041256 |
Tp53-/- proB cells, biological replicate 2 |
| GSM2041257 |
Tp53-/- proB cells, biological replicate 3 |
| GSM2041258 |
Lnk-/- proB cells, biological replicate 1 |
| GSM2041259 |
Lnk-/- proB cells, biological replicate 2 |
| GSM2041260 |
Lnk-/- proB cells, biological replicate 3 |
| GSM2041261 |
Tp53-/-Lnk-/- proB cells, biological replicate 1 |
| GSM2041262 |
Tp53-/-Lnk-/- proB cells, biological replicate 2 |
| GSM2041263 |
Leukemic Tp53-/-Lnk-/- B-ALL cells, biological replicate 1 |
| GSM2041264 |
Leukemic Tp53-/-Lnk-/- B-ALL cells, biological replicate 2 |
| GSM2041265 |
Leukemic Tp53-/-Lnk-/- B-ALL cells, biological replicate 3 |
| GSM2041266 |
Leukemic Tp53-/-Lnk-/- B-ALL cells, biological replicate 4 |
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| Relations |
| BioProject |
PRJNA309083 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE76940_RAW.tar |
132.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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