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Series GSE7705 Query DataSets for GSE7705
Status Public on May 01, 2008
Title Microarray analysis of CD4+T cells in murine arthritis
Organism Mus musculus
Experiment type Expression profiling by array
Summary Although activated CD4+ T cell-driven overproduction of cytokines, especially TNF and IL1, is generally regarded as the major factor in the development of rheumatoid arthritis (RA), little is known about the precise role of CD4+ T cells in the initiation and progression of this disease. In this study, this issue was addressed using a time-course microarray analysis in a mouse model of RA, Il1rn deficient mice.No obvious cytokine gene expression changes reflecting T cell activation was observed in CD4+ T cells in the Il1rn deficient mice during the course of spontaneous arthritis. On the contrast, majority of dysregulated genes were those predominantly expressed in myeloid lineage cells, suggesting T cell reprogramming involvement in arthritis development. Distinct gene expression patterns were identified for different stages of disease, including downregulated expression of immunoglobulin heavy chain constant region genes and increased expression of inflammatory genes in the early phase, and downregulation of MHC class II genes in the late phase. The common changes occurred in both early and late phases included upregulation of Arl2bp and Mfap1, which are involved in the regulation of cytoskeletal dynamics.
Keywords: Time course
 
Overall design Two KO mice and three WT mice for each time point (1 month and 4 month) were used for this study.
 
Contributor(s) Yan J, Jiao Y, Gu W, Stuart JM
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date May 03, 2007
Last update date Feb 11, 2019
Contact name Jian Yan
E-mail(s) jyan1@utmem.edu
Phone 901-448-7830
Organization name University of Tennessee Health Science Center
Department Orthopedics Surgery
Lab Weikuan Gu
Street address 956 Court Ave
City Memphis
State/province TN
ZIP/Postal code 38163
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (10)
GSM186523 CD4+ cells IL1RN knockout 1 month rep1
GSM186524 CD4+ cells IL1RN knockout 1 month rep2
GSM186525 CD4+ cells IL1RN knockout 4 month rep1
Relations
BioProject PRJNA99423

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Supplementary file Size Download File type/resource
GSE7705_RAW.tar 33.9 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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