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Series GSE77114 Query DataSets for GSE77114
Status Public on Jan 22, 2016
Title Perivascular Progenitor Cells Derived from Human Embryonic Stem Cells Exhibit Functional Characteristics of Pericytes, and Improve the Retinal Vasculature in a Rodent Model of Diabetic Retinopathy
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Diabetic retinopathy (DR) is the leading cause of blindness in working-age people. Pericyte loss is one of the pathologic cellular events in DR, which weakens the retinal microvessels. Damages to the microvascular networks are irreversible and permanent, thus further progression of DR is inevitable. In this study, we hypothesize that multipotent perivascular progenitor cells derived from human ESCs (hESC-PVPCs) improve the damaged retinal vasculature in the streptozotocin (STZ)-induced diabetic rodent models. We describe a highly efficient and feasible protocol to derive such cells using a natural selection method without cell sorting processes. As a cellular model of pericytes, hESC-PVPCs exhibited marker expressions such as CD140B, CD146, NG2, and functional characteristics of pericytes. Following a single intravitreal injection into diabetic Brown Norway (BN) rats, we demonstrate that the cells localized alongside typical perivascular regions of the retinal vasculature, and stabilized blood-retinal barrier (BRB) breakdown. Findings in this study highlight a therapeutic potential of hESC-PVPCs in DR by mimicking the role of pericytes in vascular stabilization.
 
Overall design The molecular profiling analysis of ontological selected gene sets demonstrated that the transcripts associated with stemness markers, neural crest, neuro-ectoderm, and endoderm gene expressions.
 
Contributor(s) Kim J, Hong K, Song W, Bae D, Chung H
Citation(s) 27388242
Submission date Jan 21, 2016
Last update date Aug 13, 2018
Contact name Hyung-Min Chung
E-mail(s) stemness@gmail.com
Organization name Konkuk university school of medicine
Department Department of Medicine
Lab Stem cell research
Street address 120 Neungdong-ro, Gwangjin-gu
City Seoul
ZIP/Postal code 143-701
Country South Korea
 
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (9)
GSM2044504 human embryonic stem cell_control_rep1
GSM2044505 human embryonic stem cell_control_rep2
GSM2044506 human embryonic stem cell_control_rep3
Relations
BioProject PRJNA309455

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE77114_RAW.tar 26.2 Mb (http)(custom) TAR
GSE77114_non-normalized.txt.gz 2.7 Mb (ftp)(http) TXT
Processed data included within Sample table

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