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| Status |
Public on Feb 19, 2020 |
| Title |
Transcriptional profiles of skeletal muscle stem cells from mice of different ages, exercise status, and Ccnd1 genotypes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Voluntary exercise enhances old skeletal muscle stem cell (MuSC) function in vivo and ex vivo, dependent on upregulation of Ccnd1. To determine the transcriptional changes associated with these phenotypes, RNA-Seq was performed on MuSCs from young and old mice that had exercised or not exercised, and from young mice with MuSC-specific loss-of-function deletions in zero, one, or both alleles of Ccnd1.
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| Overall design |
To study the effects of exercise on MuSCs, 3-month-old (Y) or 18-month-old (O) C57BL6 male mice were given access to a running wheel that was locked (-Ex) or free (+Ex) for three weeks, and hindlimb MuSCs were FACS-isolated as CD31-, CD45-, Sca1-, VCAM+, and viable (DAPI-) cells. To study the effects of Ccnd1 loss in MuSCs, 3-month-old or 18-month-old C57BL6 Pax7_CreERT2; R26R_YFP; Ccnd1_+/+ (WT), Ccnd1_+/flox (HET), or Ccnd1_flox/flox (KO) mice were injected with tamoxifen to induce recombination in MuSCs, which were FACS-isolated six weeks later as YFP+, viable (DAPI-) cells. PolyA-selected RNA libraries were constructed and sequenced on a HiSeq2000, generating paired-end 101-bp reads. Each replicate represents MuSCs from an individual mouse. Y(-Ex) = young, no exercise; O(-Ex) = old, no exercise; Y(+Ex) = young, exercise; O(+Ex) = old, exercise; Y(WT) = young, Pax7_CreERT2; R26R_YFP; Ccnd1_+/+; Y(HET) = young, Pax7_CreERT2; R26R_YFP; Ccnd1_+/flox; Y(KO) = young, Pax7_CreERT2; R26R_YFP; Ccnd1_flox/flox; O(WT) = old, Pax7_CreERT2; R26R_YFP; Ccnd1_+/+.
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| Contributor(s) |
Brett JO, Ikeda M, Rando TA |
| Citation(s) |
32601609 |
| Submission date |
Jan 25, 2016 |
| Last update date |
Jun 30, 2020 |
| Contact name |
Jamie O. Brett |
| E-mail(s) |
jamie.o.brett@gmail.com
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| Organization name |
Stanford University
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| Department |
Neurology and Neurological Sciences
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| Lab |
Thomas A. Rando
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| Street address |
3801 Miranda Avenue, Building 100, Room E4-216
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| City |
Palo Alto |
| State/province |
CA |
| ZIP/Postal code |
94304 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA309678 |
| SRA |
SRP068844 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE77178_ExerciseAgingCcnd1_MuSCRNASeq.txt.gz |
2.7 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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