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Series GSE7759 Query DataSets for GSE7759
Status Public on Aug 30, 2008
Title Spatial and Temporal Analysis of Gene Expression During Growth and Fusion of the Mouse Facial Prominences
Organism Mus musculus
Experiment type Expression profiling by array
Summary Growth and patterning of the face relies on several small buds of tissue, the facial prominences, which surround the primitive mouth. Beginning around E10 of mouse development the prominences undergo rapid growth and morphogenesis. By E11.5 the medial nasal prominences are in close apposition in the midline, as are the maxillary and medial nasal prominences on either side of the developing face. Subsequently, by E12.5 the nasal and maxillary prominences fuse to form a continuous shelf at the front of the face - the primary palate. Individual prominences are associated with specific developmental processes, and this is reflected by patterns of differential gene expression that give the prominences their unique identities. Thus, only the mandibular and maxillary prominences give rise to dentition while the frontonasal prominence has a unique role in olfaction, and the mandibular prominence in taste. We used microarrays to detail the differential gene expression program in each of the mandibular, maxillary, and frontonasal prominences during the key developmental timepoints of E10.0 through E12.5.
Keywords: time course
Overall design Analysis of gene expression during growth and fusion of the facial prominences in the C57BL/6J mouse strain between embryonic (E) day 10.0 and 12.5. At the earliest timepoint, E10, only the mandibular prominence is a distinct entity that can be readily identified and dissected. The frontonasal prominence and the maxillary prominence are very small and not discrete from other components of the head such as the forebrain until E10.5. Analysis of these tissues at earlier timepoints would require laser capture and preamplification steps - techniques that were not used for the later timepoints. Thus samples were isolated from the mandibular prominence at E10.0 and from the mandibular, maxillary and frontonasal prominences of mouse embryos from E10.5 to E12.5, at 0.5 day intervals. In order to obtain sufficient sample for hybridization, each sample represents a pool of between 3 and 48 embryos depending on the timepoint. Specifically, the number of embryos were 40-48 for E10.0 (mandibular prominence only), 24-8 for E10.5, 8-9 for E11.0 and E11.5 and 3-4 for E12.0 and E12.5. Seven replicate samples were taken for each of the later five timepoints in each of the three prominences, with an additional seven samples for the mandibular E10.0 timepoint, for a total of 112 samples.
Contributor(s) Feng W, Leach SM, Phang T, Tipney HJ, Hunter LE, Geraci M, Spritz R, Williams T
Citation(s) 20016822, 28856179
Submission date May 08, 2007
Last update date Aug 02, 2019
Contact name Trevor Williams
Phone 303-724-4571
Organization name UCHSC at Fitzsimons
Department Craniofacial Biology
Lab Trevor Williams
Street address MS-8120, PO Box 6511, 12801 E 17th Ave
City Aurora
State/province CO
ZIP/Postal code 80045
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (112)
GSM187826 Mandibular E10.0 biological replicate 1
GSM187827 Mandibular E10.0 biological replicate 2
GSM187828 Mandibular E10.0 biological replicate 3
BioProject PRJNA99887

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Supplementary file Size Download File type/resource
GSE7759_RAW.tar 649.1 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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